[Objective]To compare the effects of different extraction methods on the extraction rates of menthol and essential oil from HERBA MENTHAE. [Methods]Essential oils were extracted from HERBA MENTHAE by ultrasonic extraction method,cold leaching method,steam distillation method and supercritical CO2method. Menthol content was detected by gas chromatography. [Results]Extraction rates of essential oil and menthol were 2. 43% and 1. 77%,respectively. Extraction rates by ultrasonic extraction method were 1. 34% and 1. 09%; those by cold leaching method were 1. 27% and 1. 02%. And the extraction rates by steam distillation method were 1. 15% and 0. 90%,respectively.[Conclusions]Among the four extraction methods,supercritical CO2method was the most ideal. [Objective] To compare the effects of different extraction methods on the extraction rates of menthol and essential oil from HERBA MENTHAE. [Methods] Essential oils were extracted from HERBA MENTHAE by ultrasonic extraction method, cold leaching method, steam distillation method and supercritical CO2method. [Results] Extraction rates of essential oil and menthol were 2. 43% and 1. 77%, respectively. Extraction rates by ultrasonic extraction method were 1. 34% and 1. 09%; those by And the extraction rates by steam distillation method were 1. 15% and 0. 90%, respectively. [Conclusions] Among the four extraction methods, supercritical CO2method was the most ideal .