In order to preserve virus for identifying the resistance of rice varieties against rice black-streaked dwarf disease,a simple and reliable method was developed,through which virus-free small brown planthopper (SBPH) acquired rice black-streaked dwarf virus (RBSDV) from frozen infected leaves and the virus was transmitted to healthy rice plants.The experimental results showed that SBPH could obtain RBSDV from frozen infected rice leaves and the virus could be transmitted to a susceptible rice variety.For the ability to acquire RBSDV and transmit the virus to healthy plants by SBPH,there was no significant difference between frozen infected leaves and in vitro infected leaves.The novel method could be applied to identification of rice variety resistance to rice black-streaked dwarf disease,facilitating the breeding process for rice black-streaked dwarf disease resistance.