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目的研究竹叶黄酮中的抗炎有效成分及其对不同小鼠炎症模型的药理活性作用,初步阐明竹叶黄酮抗炎的药效物质基础。方法利用小孔吸附树脂(MCI-GEL CHP20P)、聚酰胺树脂纯化、富集竹叶黄酮不同部位;建立小鼠耳廓肿胀、气囊滑膜炎、小鼠肉芽肿试验模型,研究竹叶黄酮不同部位的抗炎活性并筛选其有效抗炎成分;最后用高分辨飞行时间液质联用仪(LC-QTOF-MS/MS)对已筛选的部位进行化学成分分析。结果筛选出最佳的竹叶黄酮抗炎活性部位,并从中鉴定出7个主要的黄酮类成分。结论初步阐明了竹叶黄酮抗炎的药效物质基础,为竹叶的抗炎作用提供了详实的数据支撑,同时为其开发和利用提供了一定的科学依据。
Objective To study the anti-inflammatory active ingredients of bamboo leaf flavonoids and their pharmacological activities on different mouse models of inflammation, and to elucidate the pharmacological basis of the anti-inflammatory properties of bamboo leaf flavonoids. Methods The macroporous adsorption resin (MCI-GEL CHP20P) was used to purify the polyamide resin and enrich the different parts of the bamboo leaf flavonoids. A mouse model of auricle swelling, balloon synovitis and granuloma of mouse was established to study the effects of flavonoids Anti-inflammatory activity and screened their effective anti-inflammatory components. Finally, the chemical constituents of the screened sites were analyzed by LC-QTOF-MS / MS. Results The best anti-inflammatory activity of bamboo leaves was screened and seven main flavonoids were identified. Conclusion The pharmacodynamic basis for the anti-inflammatory properties of bamboo leaves was initially clarified, providing detailed data support for the anti-inflammatory effects of bamboo leaves and providing some scientific evidences for its development and utilization.