论文部分内容阅读
目的建立一种体外培养人脐静脉血管内皮细胞的方法,初步探讨rhNDPK-A蛋白对内皮细胞生长的影响。方法采用胶原酶对新生儿脐带静脉血管进行消化,从中分离出血管内皮细胞,体外传代培养,通过MTS/PMS法测定不同浓度的rhNDPK-A蛋白对HUVEC体外增殖的影响。结果成功获得体外培养的人脐静脉血管内皮细胞,从0 0610μg/ml到250μg/ml的rhNDPK-A蛋白对人脐静脉血管内皮细胞的增殖既没有抑制也没有促进作用。结论建立了分离培养人脐静脉血管内皮细胞的方法,rhNDPK-A蛋白不是通过抑制内皮血管细胞的增殖而抑制血管新生的。
Objective To establish a method of culturing human umbilical vein endothelial cells in vitro and investigate the effect of rhNDPK-A protein on the growth of endothelial cells. Methods The neonatal umbilical vein was digested with collagenase. The endothelial cells were isolated and cultured in vitro. The effects of different concentrations of rhNDPK-A protein on the proliferation of HUVEC were determined by MTS / PMS. Results Human umbilical vein endothelial cells were successfully obtained in vitro. The rhNDPK-A protein from 0 0610μg / ml to 250μg / ml did not inhibit or promote the proliferation of human umbilical vein endothelial cells. Conclusion The method of isolation and culture of human umbilical vein endothelial cells was established. RhNDPK-A protein did not inhibit angiogenesis by inhibiting the proliferation of endothelial cells.