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目的 :探讨普罗布考对氧化修饰低密度脂蛋白 (ox L DL )诱导的人单核细胞株 THP- 1粘附功能的影响。 方法 :分别应用流式细胞仪和β- N -乙酰氨基己糖苷酶比色法 ,观察不同浓度普罗布考对 ox L DL诱导的 THP- 1对人脐静脉内皮细胞(HUVEC)的粘附及粘附分子 CD11b和 CD5 4表达水平的影响。 结果 :普罗布考呈浓度依赖方式抑制 ox L DL诱导的 THP- 1对 HU VEC的粘附 ,并下调粘附分子 CD11b的表达 (P<0 .0 1) ,但对 CD5 4表达水平无明显影响。结论 :普罗布考除具有调脂作用外 ,尚可抑制循环血单核 -巨噬细胞与内皮细胞粘附聚集 ,具有抗炎作用
Objective: To investigate the effect of probucol on the adhesion function of human monocytic cell line THP-1 induced by ox-LDL. Methods: Flow cytometry and β - N - acetylglucosaminidase colorimetric assay were used to observe the effect of different concentrations of probucol on the adhesion of THP - 1 induced by oxLDL to human umbilical vein endothelial cells (HUVECs) Effects of adhesion molecules CD11b and CD5 4 expression levels. Results: probucol inhibited oxLDL-induced adhesion of THP-1 to HU VEC in a concentration-dependent manner and down-regulated the expression of adhesion molecule CD11b (P <0.01), but not to CD5 4 influences. CONCLUSION: Probucol inhibits the adhesion and aggregation of circulating monocyte-macrophages and endothelial cells in addition to the lipid-lowering effect and has anti-inflammatory effects