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应用间接免疫荧光法，放射免疫分析法，微量细胞培养法及斑点杂交技术，检测了２７例乙型慢性活动性肝炎（ＣＡＨ一Ｂ）患者及７例正常对照者ＣＤ＋４、ＣＤ＋８及Ｂ细胞膜上白细胞介素一２受体（ｍＩＬ－２Ｒ，ＣＤ２５抗原为其β链），各亚群细胞培养上清及病人同期血清中可溶性白细胞介素一２受体（ｓＩＬ－２Ｒ），ＣＤ＋４细胞培养上清中ＩＬ一２活性。结果：ＣＡＨ－Ｂ病人外周血ＣＤ２５抗原阳性的ＣＤ＋４、ＣＤ＋８细胞及Ｂ细胞较正常对照者减少，且ＣＤ２５抗原阳性的ＣＤ＋４、ＣＤ＋８细胞减少较Ｂ细胞明显；ＣＤ＋细胞培养上清中ＩＬ一２活性较正常人降低，并与病情的轻重、ＣＤ＋４细胞ｍＩＬ一２Ｒ的表达呈正相关；ＣＡＨ－Ｂ病人血清ｓＩＬ－２Ｒ增高，但培养上清ｓＩＬ－２Ｒ与正常对照无显著性差异。结果提示：ＣＡＨ一Ｂ病人外周血ＣＤ＋４、ＣＤ＋８及Ｂ细胞ｍＩＬ－２Ｒ表达均减弱；ＣＡＨ－Ｂ病人体内Ｔ细胞免疫应答功能受损可能较Ｂ细胞受损明显；ＣＡＨ－Ｂ病人体内ＩＬ一２产生水平低下与病情轻重有某种关联；ＣＡＨ一Ｂ患者血清中增高的ｓＩＬ一２Ｒ可能来自肝内浸润的免疫活性细胞ｍＩＬ－２Ｒβ链脱落。
Indirect immunofluorescence, radioimmunoassay, micro-cell culture and dot blot hybridization were used to detect the leukocytes in CD + 4, CD + 8 and B cell of 27 patients with chronic hepatitis B (CAH-B) and 7 normal controls Serum levels of soluble interleukin-2 receptor (sIL-2R), CD + 4 cell culture supernatant IL-2 activity. Results: CD + 4, CD + 8 cells and B cells of CD25 antigen positive cells in CAH-B patients were lower than those in normal controls, and CD25 + CD + 8 and CD25 + cells in CAH-B patients were less than those in B cells. The expression of sIL-2R in CD + 4 cells was positively correlated with the severity of disease. The level of sIL-2R in CAH-B patients was increased, but there was no significant difference between sIL-2R and normal controls. The results suggest that the expressions of CD + 4, CD + 8 and B cell mIL-2R in peripheral blood of CAH-B patients are weakened; the immune function of T cells in CAH-B patients may be impaired compared with that of B cells; CA-B There is a certain association between the low level of serum creatinine and severity of disease. The increased serum sIL-2R in serum of CAH-B patients may come from shedding of mIL-2Rβ chain in intrahepatic infiltrating immunocompetent cells.