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通过花粉管通道法将苏云金芽孢杆菌（Ｂａｃｉｌｌｕｓｔｈｕｒｉｎｇｉｅｎｓｉｓ）毒蛋白基因（Ｂｔ．ｔｏｘｉｎｇｅｎｅ）转进了小麦品种京花５号与８６Ａｌ。利用点杂交、Ｓｏｕｔｈｅｒｎ杂交和ＰＣＲ等技术鉴定了转化植株的当代和子代，证实了Ｂｔｔｏｘｉｎｇｅｎｅ的导入与对小麦染色体的整合。利用对腺嘌呤（Ａ）与胞嘧啶（Ｃ）甲基化敏感与否的限制性内切酶组（ｍｅｄｈｙｌａｔｉｏｎ－（ｕｎ）ｓｅｎｓｉｔｉｖｅｒｅｓｔｒｉｃｔｉｏｎｅｎｚｙｍｅｇｒｏｕｐ，ＭＲＥＧ）酶切和ＲＣＲ扩增（ＭＲＥＧ－ＰＣＲ），对转化子代中的Ｂｔ基因片段甲基化形式进行了研究。结果表明，整合在小麦基因组中的Ｂｔ基因的Ａ和Ｃ的甲基化形式发生了变化
The Bacillus thuringiensis toxin gene (Bt. Toxingene) was transferred into wheat cultivar Jinghua 5 and 86Al by pollen tube pathway. The present and future generations of the transformed plants were identified by dot blotting, Southern hybridization and PCR techniques, and the integration of Bttoxingene into wheat chromosomes was confirmed. Using the MREG digestion and RCR amplification (MREG-PCR) of the group sensitive to adenine (A) and cytosine (C) methylation, the transformation The methylation patterns of Bt gene fragments in progeny were studied. The results showed that the methylation patterns of A and C in the Bt gene integrated in the wheat genome changed