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目的探讨人脐血单个核细胞(human cord blood mononuclear cells,HCMNCs)移植对压力性尿失禁(stressurinary incontinence,SUI)大鼠尿道括约肌的修复和功能重建作用。方法采用反复阴道扩张的方法模拟产道损伤建立雌性SD大鼠SUI模型。40只大鼠按随机数字表法分为模拟组10只、实验组30只(移植组15只,实验对照组15只)。羟乙基淀粉沉淀加密度梯度离心的方法制备HCMNCs,并以4,6-联脒-2-苯基吲哚(DAPI)标记。模型制作成功后即在移植组近端尿道注射已标记HCMNCs 2×106个,对照组注射相同质量PBS液。检测尿动力学指标后获取近端尿道组织,观察荧光细胞的形态和分布以及尿道括约肌的变化。结果通过反复阴道扩张可建立SUI大鼠模型,HCMNCs可在受损尿道括约肌内存活、分布,移植组比较对照组尿道括约肌得以较好修复,SUI大鼠控尿能力明显改善。结论 HCMNCs可成功移植到SUI大鼠尿道括约肌,改善控尿,促进尿道括约肌的修复。
Objective To investigate the effects of human cord blood mononuclear cells (HCMNCs) transplantation on the repair and functional reconstruction of urethral sphincter in stressurinary incontinence (SUI) rats. Methods The vaginal dilation method was used to simulate the birth canal injury to establish the female SD rat SUI model. Forty rats were divided into 10 groups according to the random number table method, 30 in the experimental group (15 in the transplantation group and 15 in the experimental group). HCMNCs were prepared by hydroxyethyl starch precipitation and density gradient centrifugation and labeled with 4,6-diamidino-2-phenylindole (DAPI). After successful model making, 2 × 106 marked HCMNCs were injected into the proximal urethra of the transplantation group, and the control group was injected with the same mass of PBS solution. The proximal urethral tissues were obtained after detecting urodynamic parameters. The morphology and distribution of fluorescent cells and the changes of urethral sphincter were observed. Results SUI rat model was established by repeated vaginal dilation. HCMNCs could survive and distribute in injured urethral sphincter. Compared with control group, urethral sphincter could be repaired in transplantation group, and urinary control ability of SUI rats improved significantly. Conclusion HCMNCs can be successfully transplanted to the urethral sphincter of SUI rats to improve urine control and promote urethral sphincter repair.