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小地老虎Agrotis ypsilon(Rottemburg)成虫飞行肌的发育常受一些因素影响而发生变化,为探讨饥饿和交配行为对飞行肌发育的影响,通过电子显微镜对雌虫飞行肌(背纵肌)的肌原纤维、线粒体结构进行观察,结果显示:4日龄饥饿雌虫,肌原纤维直径、肌节长度、肌原纤维体积均显著(P<0.05)小于取食的。7日龄饥饿雌虫肌原纤维直径、肌节长度、肌原纤维体积分数较4日龄的差异均不显著(P≥0.05),而7日龄饥饿的肌原纤维直径显著(P<0.05)大于7日龄取食的;羽化10 d后,饥饿雌虫肌节长度显著(P<0.05)大于取食雌虫的,而肌纤维体积分数和线粒体体积分数均却小于后者。7、10、13日龄交配雌虫肌原纤维横切直径分别显著(P<0.05)小于同日龄非交配的;7、10、13日龄交配雌虫肌原纤维体积分数显著(P<0.05)小于非交配的,线粒体体积分数虽然无差异(P≥0.05),但是交配雌虫的早在4日龄便已明显(P<0.05)减小。上述结果表明:正常取食的小地老虎飞行肌4日龄后会发生降解现象;饥饿抑制飞行肌前期发育和中期的降解,而促进成虫末期肌原纤维的分解;交配能促进飞行肌的降解。
The development of flight muscle of Agrotis ypsilon (Rottemburg) was often influenced by some factors. In order to investigate the effect of starvation and mating behavior on flight muscle development, the effects of starvation and mating behavior on flight muscle development were studied by electron microscopy. Fibrillar and mitochondrial structures were observed. The results showed that the 4-day starved females had significantly less myofibrillar diameter, sarcomere length and myofibrillar volume (P <0.05) than feeding. There was no significant difference in myofibril diameter, sarcomere length and myofibrillar volume fraction between the 4-day-old and 7-day-old starved females (P> 0.05) ) Was greater than 7-day-old feeding. After emergence for 10 days, the slender length of starved females was significantly (P <0.05) greater than that of female females, while the muscle fiber volume fraction and mitochondrial volume fraction were both less than the latter. The cross-sectional diameters of myofibrilla of mating females at 7, 10 and 13 days were significantly (P <0.05) less than that at the same age, respectively. The myofibrillar volume fraction of mating females at 7, 10 and 13 days was significantly (P <0.05 ) Was smaller than non-mating, although there was no difference in mitochondrial volume fraction (P≥0.05), but as early as 4 days of age, the mating females had a significant (P <0.05) decrease. The above results showed that the degradation of flight muscle of the Tigers could be degraded after 4 days of age. Starvation inhibited the pre-development and mid-term degradation of flight and promoted the breakdown of myofibrils in late-stage adults. Mating could promote the degradation of flight muscle .