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目的：探讨冰点前０～４°С低温水对机体免疫功能的影响，观察小鼠在该温度冷浴前后外周血Ｔ细胞α－醋酸萘酯酶（ＡＮＡＥ）活性变化情况。方法：将小鼠２０只随机分为观察组和对照组。在０～４°С和２５°С低温水中分别浸浴２分钟，浸浴后于１，５，２４小时采集小鼠外周血，用ＡＮＡＥ染色法测定各时相ＡＮＡＥ阳性率。结果：冷浴后１及５小时ＡＮＡＥ阳性率与浴前差异无显著性（Ｐ＞０．０５），冷浴后２４小时ＡＮＡＥ阳性率明显增高（Ｐ＜０．００１），对照组各时相ＡＮＡＥ活性无显著变化。结论：实验提示０～４°С适宜时间的冷水浴，可激发小鼠机体免疫功能。据此推测，经过一定周期冷浴训练的小鼠可能会明显提高非特异性免疫功能水平。
OBJECTIVE: To investigate the effect of low temperature water (0-4 ° C) before freezing on immune function in mice and to observe the change of the activity of α-acetate-Naphthyl Acetate (ANAE) in peripheral blood T cells before and after the cold bath. Methods: Twenty mice were randomly divided into observation group and control group. The rats were immersed in 0 ~ 4 ° С and 25 ° С low temperature water for 2 minutes, respectively. Peripheral blood was collected at 1, 5 and 24 hours after bathing. The positive rate of ANAE in each phase was determined by ANAE staining. Results: The positive rate of ANAE at 1 hour and 5 hours after cold bath was not significantly different from that before bath (P> 0.05). The positive rate of ANAE at 24 hours after cold bath was significantly higher (P <0.001) No significant change in ANAE activity. Conclusion: The experiment suggests that 0 ~ 4 ° С cold water bath, can stimulate immune function in mice. According to this speculation, after a certain period of cold bath training mice may significantly improve the level of non-specific immune function.