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目的:优选三七叶总皂苷的酶解工艺并评价其酶解产物的抗抑郁活性。方法:以人参皂苷类成分的转化率为指标,通过单因素试验优选三七叶总皂苷的酶解工艺。建立小鼠慢性温和性不可预知应激模型评价酶解产物的抗抑郁活性。结果:选择蜗牛酶为转化酶,酶解pH 4.5,温度50℃,时间12 h,人参皂苷类成分的转化率达50%。小鼠建模6周后,呈现出体重下降、快感缺失、行动迟缓等抑郁症相似行为,表明建模成功。第7~9周给药期内给药组小鼠体重及糖水偏好率显著增加,自主活动水平亦有一定程度的改善。第9周时与模型组相比,酶解产物不同(低、中、高)剂量组、氟西汀组小鼠的体重分别增加了9.33%,12.44%,18.13%,15.03%;糖水偏好率分别提高了22.64%,19.87%,19.38%,20.85%;自主活动水平分别提高了8.35%,41.30%,13.33%,12.06%,其中酶解产物中剂量组呈现极显著性差异。结论:三七叶总皂苷酶解产物对抑郁小鼠模型具有良好的抗抑郁作用。
Objective: To optimize the enzymatic hydrolysis process of total saponins of Panax notoginseng and evaluate its antidepressant activity. Methods: The ginsenoside component of the conversion rate as an indicator, through the single factor experiment optimization of the Panax notoginseng saponin enzymatic hydrolysis process. The model of chronic unpredictable stress in mice was established to evaluate the antidepressant activity of the hydrolyzate. Results: The snail enzyme was selected as invertase. The enzymatic hydrolysis was carried out at pH 4.5, temperature 50 ℃ and time 12 h. The conversion of ginsenosides reached 50%. Six weeks after modeling, mice showed the similar behavior of depression, such as weight loss, loss of pleasure and slowness of action, indicating that the modeling was successful. During the 7th to 9th week, the body weight and the preference of sugar in the medication group increased significantly and the degree of voluntary activity also improved to a certain extent. Compared with the model group, the body weight of fluoxetine-treated mice increased by 9.33%, 12.44%, 18.13% and 15.03% at the different dosage groups (low, medium and high) Respectively, increased by 22.64%, 19.87%, 19.38% and 20.85% respectively. The level of autonomous activity increased by 8.35%, 41.30%, 13.33% and 12.06% respectively. The middle dose group of the hydrolyzate showed extremely significant difference. Conclusion: The total saponin of Panax Notoginseng hydrolyzate has a good antidepressant effect on depressive mice model.