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本研究以高纤维强度材料0-153和转基因抗虫棉sGK9708为亲本构建的高代重组自交系群体(F6:8)中纤维强度具有显著差异的两个系(高强材料69307和低强材料69362)作为材料,利用cDNA芯片技术,对纤维次生壁加厚阶段(15DPA和20DPA)的基因表达谱进行研究。共检测到差异表达基因3383个,占cDNA芯片基因序列的11.59%;并从中选择4个代表性基因利用荧光定量PCR进行验证,其表达模式与芯片数据一致,证明芯片结果可靠。通过次生壁加厚期标志基因的表达模式研究发现,两个材料纤维发育过程不同步,高强纤维材料比低强纤维材料晚进入次生壁加厚期。利用Blast2GO软件对差异表达基因进行富集分析表明,在15DPA到20DPA,寡糖或多糖等碳水化合物、类苯基丙烷和类黄酮合成等次生代谢、氧化还原、细胞信号转导等生物学过程中相关基因出现显著性富集。而且,这些差异表达基因在两个材料中的表达模式具有时间差异性。这种表达模式的差异可能是影响棉纤维强度性状的重要原因之一。本研究为棉纤维次生壁加厚相关基因的克隆奠定了基础,同时也为深入挖掘棉纤维品质性状重要基因,并将其用于棉纤维品质性状的改良奠定了基础。
In this study, two lines with significant differences in fiber strength (F6: 8 and F6: 8) were constructed with high fiber strength material 0-153 and transgenic insect-resistant cotton sGK9708 as their parents (high-strength material 69307 and low- 69362) as a material, cDNA microarray technology was used to investigate the gene expression profiling of fiber secondary wall thickening stages (15 DPA and 20 DPA). A total of 3383 differentially expressed genes were detected, which accounted for 11.59% of the cDNA sequence. Four representative genes were selected and verified by real-time PCR. The expression pattern was consistent with the chip data, which proved the chip was reliable. Through the secondary wall thickening of the marker gene expression pattern study found that the two materials fiber development process is not synchronized, high-strength fiber material than the low-strength fiber material into the secondary wall thickening period. The enrichment analysis of differentially expressed genes using Blast2GO software showed that in the 15DPA to 20DPA, oligosaccharides or polysaccharides and other carbohydrates, phenylpropane and flavonoid synthesis, such as secondary metabolism, redox, cell signaling and other biological processes Related genes appear significant enrichment. Moreover, the expression patterns of these differentially expressed genes in the two materials were time-differentiated. This difference in expression pattern may be one of the important reasons that affect the cotton fiber strength traits. This study laid the foundation for the cloning of genes related to secondary thickening of cotton fiber, and laid the foundation for further digging of important genes of cotton fiber quality traits and their application to the improvement of cotton fiber quality traits.