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【目的】比较15°C、20°C和25°C时,9株不同致病性与非致病性副溶血性弧菌菌株在TSB(3%NaCl,pH 8.0)中的最大比生长速率之间的差异。【方法】应用Bioscreen C全自动微生物生长曲线分析仪测定副溶血性弧菌的最大比生长速率。【结果】15°C、20°C和25°C时,9株副溶血性弧菌菌株间最大比生长速率的变异系数分别为20.72%、17.5%和15.98%。不同副溶血性弧菌最大比生长速率之间的差异随着温度的降低而增加。【结论】在进行定量微生物风险评估时,应用仅基于一株菌建立的生长预测模型会给预测结果带来较大的不确定性。需建立可描述不同副溶血性弧菌菌株的最大比生长速率的随机模型来为定量微生物风险评估提供更准确有效的预测模型。
【OBJECTIVE】 To compare the maximal specific growth rate of nine pathogenic and non-pathogenic Vibrio parahaemolyticus strains in TSB (3% NaCl, pH 8.0) at 15 ° C, 20 ° C and 25 ° C difference between. 【Method】 The maximum specific growth rate of Vibrio parahaemolyticus was determined by Bioscreen C automatic microbial growth curve analyzer. 【Result】 The coefficients of variation (CV) of the maximum specific growth rate among strains of Vibrio parahaemolyticus at 15 ° C, 20 ° C and 25 ° C were 20.72%, 17.5% and 15.98%, respectively. The difference between the maximum specific growth rate of Vibrio parahaemolyticus increased with decreasing temperature. 【Conclusion】 The application of the growth prediction model based on only one strain of bacteria will bring greater uncertainty to the prediction results in quantitative microbial risk assessment. A stochastic model describing the maximum specific growth rate of different V. parahaemolyticus strains should be established to provide a more accurate and effective prediction model for quantitative microbial risk assessment.