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以 PCR、免疫荧光、细胞毒试验在 DNA、表达、功能水平对转 h DAF基因猪进行了检测。结果表明 :转基因猪的整合率为 19.35 % ( 18/ 93) ;在 14头转基因阳性猪中 ,有 7头动脉肌肉层表达了外源 h DAF基因 ,6头猪的外源基因表达产物可以抑制猪淋巴细胞的裂解
PCR, immunofluorescence and cytotoxicity assays were performed on pigs transfected with h DAF gene at the level of DNA, expression and function. The results showed that the integration rate of transgenic pigs was 19.35% (18/93). Exogenous h DAF gene was expressed in 7 arterial muscle layers of 14 transgenic pigs and the exogenous gene expression products of 6 pigs could be inhibited Lysis of porcine lymphocytes