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目的 :探讨不同强度应激对小鼠衰老改变影响的差异。方法 :用D 半乳糖进行衰老诱导的小鼠同步进行不同水温 (12℃、18℃、2 5℃ )游泳应激训练 ,连续六周后 ,进行开场行为、学习记忆能力、半脑MDA含量及海马SOD活性测定。结果 :D 半乳糖诱导组 (D)较对照组 (C)探究行为减少 ,获得性行为能力减退 (P <0 .0 1) ,且脑内脂褐素含量明显增加 (P <0 .0 5 ) ;18℃及 2 5℃水温应激训练有对抗D 半乳糖所致行为和学习记忆能力减退的作用 (P <0 .0 1) ,同时脑中抗氧化能力提高 (P <0 .0 5 ) ,而 12℃水温组学习记忆能力进一步减退 ,海马SOD活性进一步下降(P <0 .0 5 ) ,且各项行为能力及SOD活性与 18℃和 2 5℃组均有显著差异 (P <0 .0 1)。结论 :不同水温游泳应激对机体的影响具有双重性 ,适量的应激刺激可逆转机体的衰老改变 ,提高动物的学习记忆能力 ,而过强的应激刺激有加速衰老进程的倾向。两种应激对机体抗氧化能力的不同改变可能是引起这一差异的原因。
Objective: To explore the different intensity of stress on aging changes in mice. Methods: Mice induced by aging with D-galactose were subjected to swimming stress training at different water temperatures (12 ℃, 18 ℃, 25 ℃) simultaneously. After six consecutive weeks, the opening behavior, learning and memory ability, MDA content in hemisphere and Determination of SOD activity in hippocampus. Results: Compared with the control group (C), D-galactose-induced group (D) had less probing behavior and acquired sexual behavior ability (P <0.01), and the lipofuscin content in the brain increased significantly (P <0.05) ); 18 ℃ and 25 ℃ water temperature training had anti-D galactose-induced behavioral and learning and memory abilities (P <0.01), while the brain antioxidant capacity increased (P <0. 05 ). However, the learning and memory abilities of 12 ℃ water temperature group decreased further, the activity of SOD in hippocampus decreased further (P <0.05), and all the activities and SOD activities were significantly different from those in 18 ℃ and 25 ℃ groups (P < 0 .0 1). Conclusion: The effects of swimming stress at different water temperatures on the body are dual. The appropriate amount of stress stimulation can reverse the senescence of the body and enhance the learning and memory abilities of animals. However, excessive stress stimulates the aging process. Two different stress on the body’s antioxidant capacity may be the cause of this difference.