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目的比较研究黄皮叶不同提取物的降脂效果,并探讨其对非酒精性脂肪肝(NAFLD)大鼠降脂保肝作用及机制。方法制备黄皮叶水提物和醇提物,建立小鼠高脂模型,测定高脂模型小鼠血清中的血脂成分。选择作用较强的黄皮叶醇提物,采用灌饲高脂肪乳剂方法制备高脂性脂肪肝大鼠模型,观察其对血清中血脂、肝酶活性及肝脏组织病理学改变的影响。结果黄皮叶水提物及醇提物均能降低高血脂小鼠血清胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白胆固醇(LDL-C)含量,升高高密度脂蛋白胆固醇(HDL-C)含量,醇提物比水提物作用强。黄皮叶醇提物能明显降低高脂性脂肪肝大鼠血清中TC、TG、LDL-C及丙氨酸氨基转移酶(ALT),天冬氨酸氨基转移酶(AST)的含量,减轻肝脏的病理损害。结论黄皮叶醇提取物降脂效果优于水提物,且具有良好的降脂保肝作用。
Objective To compare the lipid-lowering effects of different extracts from Huangpi leaf and to explore the mechanism of its lipid-lowering and hepatoprotective effects on non-alcoholic fatty liver disease (NAFLD) rats. Methods The aqueous extract of Huangpiye and alcohol extract were prepared, the model of hyperlipidemia in mice was established, and the content of lipids in the serum of model mice was determined. The alcoholic extract of Huangpi leaf with stronger effect was selected to prepare the rat model of fatty liver caused by hyperlipidemia by using high fat emulsion. The effects on serum lipids, liver enzymes and liver histopathological changes were observed. Results Both water extract and alcohol extract of Huangpiye could decrease the contents of serum cholesterol (TC), triglyceride (TG) and low density lipoprotein cholesterol (LDL-C) and increase the content of high density lipoprotein cholesterol (HDL-C) content, alcohol extract stronger than water extract. Alcohol extract of Huangpi leaf can significantly reduce the content of TC, TG, LDL-C, ALT and AST in the serum of the rats with fatty liver and relieve the liver The pathological damage. Conclusion The lipid-lowering effect of the extract of Huangpiye is better than that of the water extract, and has a good lipid-lowering and hepatoprotective effect.