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目的分析南宁市腹泻患者非伤寒沙门菌的血清型分布和药敏特征,为临床用药和制定防控策略提供参考。方法根据WS 271—2007《感染性腹泻诊断标准》,进行菌株的鉴定和血清分型;采用微量肉汤法进行药物敏感性试验,根据最低抑菌浓度(MIC)进行药敏结果分析和判读。结果南宁市腹泻患者非伤寒沙门菌分离株以B群为优势群(62/86,72.09%),鼠伤寒沙门菌(31.40%)、德尔卑沙门菌(16.28%)为主要血清型。86株菌对碳青霉烯类抗生素亚胺培南100%敏感,对其他类型的抗生素均有一定程度的耐药,对磺胺甲恶唑的耐药率最高,达76.74%,对四环素类抗生素、青霉素类抗生素的耐药率达55%。菌株的总体耐药率为87.21%(75/86),多重耐药现象严重,4种及4种以上抗生素的耐药率为58.14%(50/86),不同血清型的4重及以上多重耐药率差异无统计学意义(χ2=1.032,P>0.05)。结论南宁市引起腹泻的非伤寒沙门菌血清型众多,耐药严重,需继续加强耐药监测,同时共同努力规范抗生素的使用。
Objective To analyze the serotype distribution and drug sensitivity of non-typhoid Salmonella in patients with diarrhea in Nanning City, and to provide reference for clinical medication and prevention and control strategies. Methods According to WS 271-2007 Diagnostic Criteria of Infectious Diarrhea, the strains were identified and serotyped. The drug susceptibility test was carried out by using micro broth method. The susceptibility results were analyzed and interpreted according to the minimum inhibitory concentration (MIC). Results Non-Salmonella Salmonella isolates from patients with diarrhea in Nanning City were dominated by group B (62/86, 72.09%), Salmonella typhimurium (31.40%) and Salmonella delphi (16.28%). 86 strains of carbapenem antibiotics imipenem 100% sensitive to other types of antibiotics have a certain degree of resistance, the highest rate of sulfamethoxazole, up to 76.74%, tetracycline antibiotics , Penicillin antibiotic resistance rate of 55%. The overall drug resistance rate of the strains was 87.21% (75/86), the multi-drug resistance was serious, the drug resistance rate of 4 and more antibiotics was 58.14% (50/86), and the multiple serotypes were 4 and more There was no significant difference in drug resistance rate (χ2 = 1.032, P> 0.05). Conclusions There are many serotypes of non-typhoid Salmonella causing diarrhea in Nanning City, and there are many serotypes of drug resistance. Therefore, it is necessary to continue to strengthen drug resistance surveillance and work together to standardize the use of antibiotics.