在新方法的发展过程当中,色谱方法最重要的目标之一是得到具有一致性且可重现的分离。选取具有高重现性和良好性能的高效液相色谱(HPLC)柱是实现这一目标最基本的要素。对装入HPLC柱的色谱介质进行物理测试毋庸置疑也是非常重要的,该测试可以揭示HPLC柱的部分色谱特性。柱的本身特性以及如何与一个宽范围的分析物相互作用只能通过严格的色谱评估来获得。 One of the most important goals of the chromatographic method in developing new methods is to obtain consistent and reproducible separations. High performance liquid chromatography (HPLC) columns with high reproducibility and good performance are the most essential elements to achieve this goal. It is undoubtedly and critically important to conduct physical testing of chromatographic media loaded into the HPLC column, which will reveal part of the chromatographic properties of the HPLC column. The nature of the column itself and how it interacts with a wide range of analytes can only be obtained by rigorous chromatographic evaluations.