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目的研究蛇床子素对大鼠局灶性脑缺血再灌注损伤的保护作用。方法利用大鼠大脑中动脉短暂阻塞(MCAO)造成局灶性脑缺血(2h)再灌注(24h)损伤模型,缺血后1h舌下iv给予蛇床子素5、10mg/kg,再灌注24h,检测大鼠神经功能行为缺陷评分、脑水肿和脑梗死范围,测定脑组织中Na+,K+-ATP酶、Ca2+-ATP酶的活性和髓过氧化物酶(MPO)的活性及用放免法测定脑组织中白细胞介素-8(IL-8)的量。结果蛇床子素能改善大鼠脑缺血再灌注后神经功能行为缺陷评分,减轻脑水肿和降低脑梗死范围,增强Na+,K+-ATP酶和Ca2+-ATP酶活性,降低脑组织中MPO的活性和IL-8的量。结论蛇床子素对脑缺血再灌注损伤有保护作用,其机制可能与蛇床子素抑制大鼠脑缺血再灌注后炎症反应和减轻脑水肿有关。
Objective To study the protective effect of osthole on focal cerebral ischemia-reperfusion injury in rats. Methods The rats were subjected to focal cerebral ischemia (2h) reperfusion (24h) injury induced by transient occlusion of middle cerebral artery (MCAO) The neurobehavioral deficits, cerebral edema and cerebral infarction were measured. The activities of Na +, K + -ATPase, Ca2 + -ATPase and myeloperoxidase (MPO) in brain tissue were measured and the activity of MPO was determined by radioimmunoassay The amount of interleukin-8 (IL-8) in brain tissue. Results Osthole could improve neurological deficit score, reduce brain edema and cerebral infarction area, increase Na +, K + -ATPase and Ca2 + -ATPase activity and decrease MPO activity in brain tissue after cerebral ischemia-reperfusion And the amount of IL-8. Conclusion Osthole can protect against cerebral ischemia-reperfusion injury. Its mechanism may be related to the inhibition of osmotic reaction and cerebral edema by osthole after cerebral ischemia-reperfusion in rats.