论文部分内容阅读
目的探讨过敏性紫癜及紫癜性肾炎患儿胰岛素样生长因子-1(IGF-1)变化的临床意义。方法选择过敏性紫癜(HSP组)患儿31例和紫癜性肾炎(HSPN组)患儿28例及健康对照儿童(正常对照组)31例,分别采集血清,用酶联免疫吸附测定法(ELISA)法测定各组血清标本的IGF-1浓度,HSPN组检测24h尿蛋白定量。结果 (1)正常对照组、HSP组、HSPN组血清IGF-1水平分别为(0.272 2±0.107 1)、(0.599 9±0.346 8)、(0.906 0±0.346 8)ng/L,3组间比较差异有统计学意义(F=22.98,P<0.05),HSPN组血清IGF-1水平高于HSP组和正常对照组(P均<0.05)。(2)在12例进行肾穿刺活检的HSPN患儿中,血清IGF-1表达水平最高可达1.376ng/L,最低为0.198ng/L,随着HSPN的病理分级加重,血清IGF-1水平有增高趋势。(3)有蛋白尿的HSPN患儿的血清IGF-1水平高于无蛋白尿的HSPN患儿(P<0.05),有血尿和无血尿的HSP患儿血清中IGF-1水平差异无统计学意义(P>0.05)。(4)HSPN组患儿随尿蛋白水平的增加,血清IGF-1表达水平逐渐增加(P<0.05)。结论在HSP急性发作期可以观测到增加的IGF-1,其水平的提高可能与蛋白尿的水平有关,并可作为预测早期肾小球和肾小管损害的指标。IGF-1水平可能是一个肾脏受累的指标。
Objective To investigate the clinical significance of insulin-like growth factor-1 (IGF-1) in children with Henoch-Schonlein purpura and purpuric nephritis. Methods Thirty-one children with Henoch-Schonlein purpura (HSP) and 28 children with HSPN (HSPN group) and 31 healthy control children (control group) were enrolled in this study. Serum was collected and analyzed by enzyme-linked immunosorbent assay ) Method was used to determine the serum IGF-1 concentration in each group, 24h HSPN group quantitative urinary protein. Results The serum levels of IGF-1 in normal control group, HSP group and HSPN group were (0.272 2 ± 0.107 1), (0.599 9 ± 0.346 8) and (0.906 0 ± 0.346 8) ng / L, respectively The difference was statistically significant (F = 22.98, P <0.05). The serum IGF-1 level in HSPN group was higher than that in HSP group and normal control group (all P <0.05). (2) The expression of IGF-1 in serum of 12 HSPN patients with renal biopsy was up to 1.376ng / L and the lowest was 0.198ng / L. With the increase of HSPN pathological grade, the serum IGF-1 level Increasing trend. (3) Serum IGF-1 level in HSPN children with proteinuria was significantly higher than that in HSPN children without proteinuria (P <0.05). There was no significant difference in serum IGF-1 levels between children with and without hematuria Significance (P> 0.05). (4) The level of serum IGF-1 in HSPN group increased gradually with the increase of urinary protein level (P <0.05). Conclusions Increased IGF-1 levels can be observed during the acute exacerbation of HSP, which may be related to the level of proteinuria and may be used as an indicator of early glomerular and tubular damage. IGF-1 levels may be an indicator of kidney involvement.