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目的:探讨EBV相关胃癌(EBV associated gastric carcinoma, EBVaGc)病毒基因表达、Bcl-2蛋白表达与细胞凋亡的相互关系,明确其在EBVaGC发生发展中的作用. 方法:选取EBVaGC和相应癌旁组织13例,与之匹配的EBVnGC 45例,采用TUNEL法及免疫组化技术检测其细胞凋亡指数(AI)和bcl-2蛋白的表达;RT-PCR-Southern杂交技术检测EBV相关基因表达. 结果:EBVaGC、EBVnGC及EBVaGC癌旁组织中AI分别为0.97±0.41,2.03±0.60和3.25±0.46,统计学分析表明: EBVaGC和EBVnGC以及EBVaGC癌组织和相应癌旁组织细胞凋亡指数均有显著性差异(t=5.9795,P=0;t=13.2229, P=0).EBVaGC组和EBVnGC组Bcl-2阳性率分别为53.8% 和48.9%,两组间无显著性差异(X2=0.0991,P=0.7529). EBVaGC 13例EBNA1 mRNA均阳性,而EBNA2和LMP1 mRNA均阴性;EBV早期基因BARF1表达阳性6例,BHRF1 表达阳性2例. 结论:EBVaGC癌组织Bcl-2表达与EBV感染无明显相关性,EBV感染抑制细胞凋亡不是上调Bcl-2表达而实现, EBV早期基因BARF1和BHRF1可通过抑制细胞凋亡和细胞转化作用参与EBVaGC的发生.
OBJECTIVE: To investigate the relationship between the expression of EBV-associated gastric carcinoma (EBV-associated gastric carcinoma) and the expression of Bcl-2 and apoptosis in EBV-associated gastric carcinoma (EBVaGc) .Methods: EBVaGC and its corresponding paracancerous tissues 13 cases matched with EBVnGC, 45 cases were detected by TUNEL method and immunohistochemical detection of apoptosis index (AI) and bcl-2 protein expression; RT-PCR-Southern hybridization detection of EBV-related gene expression.Results : The AI of EBVaGC, EBVnGC and EBVaGC adjacent tissues were 0.97 ± 0.41, 2.03 ± 0.60 and 3.25 ± 0.46, respectively. Statistical analysis showed that the apoptotic index of EBVaGC, EBVnGC and EBVaGC cancer tissues and corresponding paracancerous tissues were significant The positive rates of Bcl-2 in EBVaGC group and EBVnGC group were 53.8% and 48.9%, respectively, with no significant difference between the two groups (χ2 = 0.0991, P = 0; t = 13.2229, P = = 0.7529). EBVaGC 13 cases of EBNA1 mRNA were positive, while EBNA2 and LMP1 mRNA were negative; EBV early gene BARF1 expression in 6 cases, BHRF1 expression in 2 cases.Conclusion: EBVaGC cancer Bcl-2 expression and EBV infection was not significantly correlated EBV infection inhibited apoptosis is not up-regulated Bcl-2 expression and achieve, EBV early genes BARF1 and BHRF1 can participate in the occurrence of EBVaGC by inhibiting apoptosis and cell transformation.