论文部分内容阅读
成功制备了Fe3O4磁性纳米颗粒及二甲基丙烯酸乙二醇酯-甲基丙烯酸(EGDMA-MAA)共聚物包覆的Fe3O4磁性复合微球。将吲哚美辛抗体固定在复合微球表面,形成了Fe3O4(核)/聚合物-抗体(壳)的复合免疫磁性颗粒。XRD结果表明,制备的Fe3O4的晶型为反立方尖晶石型且纯度较高;TEM表征表明Fe3O4粒径较为均匀,平均粒径为12nm;磁性复合微球的平均直径为460nm。制备的Fe3O4磁性纳米颗粒和磁性复合微球有较强的磁响应强度,其饱和磁化率分别为49.16和8.38emu/g,能够满足磁性分离的要求。FT IR验证了磁性复合微球中羧基特征峰的存在,表明羧基成功连接在磁性微球上面。通过碳二亚胺/N-羟基琥珀酰亚胺(EDC/NHS)活化法将微球表面羧基活化并成功与抗吲哚美辛抗体交联。
The Fe3O4 magnetic composite microspheres coated with Fe3O4 magnetic nanoparticles and ethylene glycol dimethacrylate (EGDMA-MAA) copolymer were successfully prepared. The indomethacin antibody was immobilized on the surface of the composite microspheres to form composite immunomagnetic particles of Fe3O4 (core) / polymer-antibody (shell). XRD results show that the prepared Fe3O4 crystal form is anti-cubic spinel type and high purity; TEM characterization shows that the Fe3O4 particle size is more uniform, the average particle size is 12nm; the average diameter of the magnetic composite microspheres is 460nm. The prepared Fe3O4 magnetic nanoparticles and magnetic composite microspheres have strong magnetic response strength, the saturation magnetic susceptibility was 49.16 and 8.38emu / g, respectively, to meet the requirements of magnetic separation. The FT IR verified the existence of carboxyl characteristic peaks in the magnetic composite microspheres, indicating that the carboxyl groups were successfully attached to the magnetic microspheres. Carboxyl groups on the surface of microspheres were activated by carbodiimide / N-hydroxysuccinimide (EDC / NHS) and successfully cross-linked with anti-indomethacin antibodies.