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目的观察外周血中性粒细胞CD64的表达水平,探讨其在新生儿败血症诊断中的临床意义。方法选择2012年1月至2013年2月在新生儿重症监护室(NICU)住院的79例新生儿,根据患儿的高危因素、住院期间的临床症状、体征及实验室检测分为新生儿败血症组(25例)、一般感染组(26例)及非感染组(28例)。应用流式细胞仪测定外周血中性粒细胞CD64表达,并探讨其意义。结果败血症组治疗前外周血中性粒细胞CD64表达高于一般感染组及非感染组(P<0.05,P<0.01);治疗7 d后,败血症组及一般感染组中性粒细胞CD64表达均显著低于治疗前(P均<0.05),但败血症组中性粒细胞CD64表达仍然高于一般感染组及非感染组(P均<0.05)。结论外周血中性粒细胞CD64表达水平可以作为早期诊断新生儿败血症的理想指标,为可疑败血症的高危儿提供实验依据。
Objective To observe the expression of CD64 in peripheral blood neutrophils and to explore its clinical significance in the diagnosis of neonatal sepsis. Methods From January 2012 to February 2013, 79 newborns hospitalized in Neonatal Intensive Care Unit (NICU) were divided into neonatal sepsis according to the risk factors of children, clinical symptoms and signs during hospitalization, and laboratory tests. (25 cases), general infection group (26 cases) and non-infected group (28 cases). The expression of CD64 in peripheral blood neutrophils was measured by flow cytometry and its significance was discussed. Results The expression of CD64 in peripheral blood neutrophil before treatment in sepsis group was higher than that in normal infection group and non-infected group (P <0.05, P <0.01). After 7 days of treatment, CD64 expression of neutrophils in sepsis group and general infection group (P <0.05). However, the expression of neutrophil CD64 in sepsis group was still higher than that in normal and non-infected patients (all P <0.05). Conclusion The expression of CD64 in peripheral blood neutrophils can be used as an ideal indicator for the early diagnosis of neonatal sepsis and provide experimental evidence for high risk children with suspected sepsis.