建立采用高效液相色谱法测定5-FPE脂质体包封率的方法。色谱柱为Kromasil 100 A C18column(350mm×4.6mm,5μm),流动相为甲醇-水(58:42,v/v),流速为0.8mL/min,测定波长为271nm,进样量为10μL,柱温为30oC。线性范围为0.8～12.8μg/mL,相关系数r=0.9999,日内和日间精密度的RSD小于2.97%,平均回收率为96.8%～104.6%,RSD小于2.24%。本文建立了采用高效液相色谱测定5-FPE脂质体包封率的方法,该方法简便、快速、精确、灵敏度高。 A method for determining the entrapment efficiency of 5-FPE liposomes by HPLC was established. The chromatographic column was Kromasil 100 A C18column (350 mm × 4.6 mm, 5 μm). The mobile phase was methanol-water (58:42, v / v), the flow rate was 0.8 mL / min, the measurement wavelength was 271 nm, The column temperature is 30oC. The linear range was from 0.8 to 12.8 μg / mL and the correlation coefficient was 0.9999. The RSD of intra- and inter-day precision was less than 2.97%. The average recovery was 96.8% -104.6%. The RSD was less than 2.24%. In this paper, a method for determining the entrapment efficiency of 5-FPE liposomes using high performance liquid chromatography has been established. The method is simple, rapid, accurate and sensitive.