利用荧光显微镜及电子显微镜观察经过处理的白萝卜自花授粉花期和蕾期柱头,发现花粉粒在柱头上的萌发及花粉管在柱头表面和花柱中的生长情况有很大差异。白萝卜自交不亲和系06074花期有少量的花粉粒萌发,并在柱头表面发生反应且花粉管的形态不正常,不能穿越乳突细胞壁;而蕾期柱头上,花粉粒几乎都能萌发。另外,老龄植株花的亲和性比幼龄植株花稍高。 Fluorescent microscopy and electron microscopy were used to observe the self-pollination flowering stage and bud stage stigma of the white radish. The results showed that the pollen grains germinated on the stigma and the growth of pollen tube on the stigma surface and style was very different. White radish self-incompatibility line 06074 at the flowering stage a small amount of pollen grains germination, and the stigma surface reaction and the morphology of pollen tube is not normal, can not cross the mast cell wall; and bud stage stigma, pollen almost germination. In addition, the flowers of older plants have slightly higher affinity than young plants.