The formation depth of metamorphic rocks in the Dabie ultrahigh pressure metamorphic (UHPM) zone influences not only our understanding of formation mechanism and evolution processes of collision orogenic belt, but also the studies on earth's interior and geodynamic processes. In this study, the isotopic data of metamorphic rocks in the Dabie UHPM zone are discussed to give constraints on the formation depth in the Dabie UHPM zone. The εSr of eclogite in the Dabie UHPM zone varies from 18 to 42, and the εNd varies from -6.1 to -17, both of them show the characters of isotopic disequilibrium. The oxygen isotope studies indicate that the protoliths of these UHPM rocks have experienced oxygen isotope exchange with meteoric water (or sea water) before metamorphism and no significant changes in the processes of metamorphism on their oxygen isotope composition have been recorded in these rocks. Except for one sample from Bixiling, all samples of eclogite from Dabie UHPM zone show the 3He/4He ratios from 0.79×10-7 to 9.35×10-7, indicating the important contribution of He from continental crust. All Sr, Nd, O and He isotopic studies indicate that the UHPM rocks retain the isotopic characteristics of their protoliths of crust origin. No significant influence of mantle materials has been found in these metamorphic rocks. Trying to explain above isotopic characteristics, some researchers assume that the speeds of dipping thrust and uplifting of rocks were both very high. In this condition, there will not be enough time for isotopic exchange between crust protolith and mantle materials. Therefore, we can not see the tracer of mantle materials in these UHPM rocks. However, this assumption can not be justified with available knowledge. Firstly, it was estimated that the whole process of UHPM took at least 15 Ma. During such a long period, and at the metamorphic temperature of ≥700 ℃, the protolith of crust origin can not escape from isotopic exchange with mantle materials if the UHPM have happened in the mantle depth of ≥100 km. In contrast, all problems will be dismissed if we assume that the UHPM have happened at the depth still in crust.