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目的:观察覆盆子不同提取部位对东莨菪碱、亚硝酸钠及40%乙醇造成的小鼠记忆障碍模型的影响,探索覆盆子对这3种记忆障碍模型的有效部位,为进一步研究覆盆子对学习记忆改善作用的机制提供依据。方法:昆明种小鼠130只,随机分成13组,每组10只。即正常组,模型组,阳性组,覆盆子全药高剂量和低剂量组,三氯甲烷部位高剂量和低剂量组,正丁醇部位高剂量和低剂量组,乙酸乙酯部位高剂量和低剂量组,水提部位高剂量和低剂量组。给药组小鼠分别以高、低剂量(24,12 g·kg~(-1))ig覆盆子全药、三氯甲烷部位、正丁醇部位、乙酸乙酯部位、水提部位;阳性药组给银杏叶制剂量为7.6 mg·kg~(-1),正常组和模型组给予等量的蒸馏水,连续12 d。7 d后除正常组外,东莨菪碱所致模型测试前30 min ip 1 mg·kg~(-1),亚硝酸钠所致模型训练后ip 120 mg·kg~(-1),40%乙醇所致模型测试前30 min ig 0.1 m L·kg~(-1),检测小鼠在Morris水迷宫的定位航行能力和空间探索能力。结果:与正常组比较,3种记忆障碍逃避潜伏期明显延长,穿越平台次数明显缩短(P<0.05);与模型组比较,覆盆子全药、三氯甲烷部位、正丁醇部位、乙酸乙酯部位、水提部位均可缩短3种记忆障碍逃避潜伏期,增加其穿越平台次数(P<0.05)。结论:覆盆子水提部位高剂量组对东莨菪碱、亚硝酸钠造成的小鼠记忆障碍模型改善效果最优;覆盆子全药高剂量组对40%乙醇造成的记忆障碍小鼠模型改善效果最优。
OBJECTIVE: To observe the effect of different extraction sites of raspberry on mouse model of memory impairment caused by scopolamine, sodium nitrite and 40% ethanol, and to explore the effective parts of raspberry on these three models of memory impairment. In order to further study the effect of raspberry on learning and memory Improve the mechanism of action to provide the basis. Methods: 130 Kunming mice were randomly divided into 13 groups of 10 rats. Namely, normal group, model group, positive group, high-dose and low-dose raspberry whole group, high-dose and low-dose chloroform group, high-dose and low dose of n-butanol, high dose of ethyl acetate and Low-dose group, high-dose and low-dose group. The mice in the drug-treated group were given high and low dose (24, 12 g · kg -1) of ig rape, chloroform fraction, n-butanol fraction, ethyl acetate fraction, The dosage of Ginkgo biloba was 7.6 mg · kg -1, and the normal group and the model group were given distilled water for 12 days. After 7 days, the scopolamine-induced model rats were injected intraperitoneally with ip 1 mg · kg -1 at 30 min before the model test and ip 120 mg · kg -1, 40% ethanol after training with sodium nitrite The model was operated at 0.1 m L · kg ~ (-1) 30 min before the test to determine the ability of the mice to navigate and explore the space in the Morris water maze. Results: Compared with the normal group, the escape latency of three kinds of memory impairment was significantly prolonged and the number of passing through the platform significantly shortened (P <0.05). Compared with the model group, the whole drug, chloroform, n-butanol, ethyl acetate Site and water extract could shorten the escape latency of three kinds of memory impairment and increase the number of crossing the platform (P <0.05). Conclusion: The high-dose raspberry water extract group scopolamine and sodium nitrite mouse memory impairment model to improve the best effect; raspberry whole drug high-dose group 40% ethanol-induced memory impairment in mice model to improve the best .