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目的:探讨贲门癌显微自体荧光图象的特征表现。方法:采用激光扫描共聚焦显微镜,以氩离子(Ar+)激光(EX=488nm)和氦氖激光(He-Ne)(EX=543nm)为激发光的双通道法,对12例贲门癌手术标本包括贲门癌组织与同体胃体组织进行自体荧光检测,并进行显微图象分析和自体荧光强度的比较。结果:癌细胞的高度增生和浸润使组织原有结构被破坏。贲门癌组织癌细胞浸润区域的平均自体荧光强度绿光为62·03,红光为18·50,而正常胃体组织平均荧光强度为:绿光168·06,红光139·79。与正常组织各层分别比较,癌组织的自体荧光强度均明显减弱,差异有统计学意义,P值均<0·01。结论:贲门癌与正常胃体组织的显微自体荧光无论在形态、颜色和分布上,还是在荧光强度上都存在巨大的差异。
Objective: To investigate the characteristics of microscopic autofluorescence in cardiac cancer. Methods: A total of 12 gastric cardia carcinoma specimens were obtained by laser scanning confocal microscopy and dual-channel method with Ar + laser (EX = 488nm) and He-Ne (EX = 543nm) Including cardiac cancer tissues and body tissues of autologous autofluorescence detection, and microscopic image analysis and autofluorescence intensity comparison. Results: The hyperplasia and infiltration of cancer cells destroyed the original structure of the tissue. The mean autofluorescence intensities of cancer cells in gastric cardia cancer tissues were 62.03 and 18.5, respectively. The average fluorescence intensity of normal gastric tissues was 168.6 and 153.73, respectively. Compared with the normal tissue layers, the autofluorescence intensity of cancer tissue was significantly weakened, the difference was statistically significant (P <0.01). Conclusion: There is a huge difference in the autofluorescence of gastric cardia carcinoma and normal gastric tissue in terms of morphology, color and distribution, and fluorescence intensity.