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目的 探讨溶栓合剂的超早期颈内动脉溶栓疗效。方法 采用改进的微栓子法建立大鼠局灶脑缺血模型 ,缺血后 3h颈内动脉逆向插管溶栓治疗。将模型鼠随机分为A、B、C 3组 (A组为生理盐水对照组、B组为尿激酶治疗组、C组为溶栓合剂治疗组 ) ,分别在缺血后 2h、溶栓后 2 5h和 2 3 5h评定神经功能 ,溶栓后 2 4h取脑制备HE染色光镜标本及透射电镜标本观察病理学改变。结果 (1)A、B、C 3组的神经功能评分两两相比 ,给药前无显著差异 ,给药后C组与A组相比差异有显著性 (P <0 0 5 ) ,B组与A组间差异无显著性 ;(2 )A、B、C 3组的光镜病理学分级评分结果分别为 (3 0 6± 0 93)、(2 6 4± 1 2 2 )、(2 11± 1 0 8) ,C组与A组相比差异有显著性 (P <0 0 5 ) ;B组与A组相比差别无显著性。透射电镜观察 ,A组病变最重 ,B组其次 ,C组最轻。结论 溶栓合剂能够改善大鼠脑缺血后的神经功能 ,减轻病理改变 ;尿激酶对神经功能及病理改变的影响无显著意义。
Objective To investigate the effect of thrombolysis combined with ultra-early internal carotid artery thrombolysis. Methods The model of focal cerebral ischemia in rats was established by modified microembolization method, and the intubation and thrombolysis of the internal carotid artery was performed 3 h after ischemia. The model rats were randomly divided into A, B and C groups (group A: normal saline control group, group B: urokinase treatment group and group C: thrombolytic agent treatment group), after 2h, The neurological function was evaluated at 2 5h and 2 3 5h. The brain tissue samples were collected at 24 hours after thrombolysis. Pathological changes were observed by transmission electron microscopy. Results (1) There was no significant difference in nerve function score between groups A, B and C before and after administration, there was significant difference between group C and group A (P <0.05), B There was no significant difference between group A and group A. (2) The results of pathological grading by light microscopy in group A, B and C were (3 0 6 ± 0 93), (2 64 ± 1 2 2), ( 2 11 ± 1 0 8). There was a significant difference between group C and group A (P <0 05). There was no significant difference between group B and group A. Transmission electron microscopy showed that the lesions in group A were heaviest, group B was the second, and group C was the lightest. Conclusion: Thrombolytic agent can improve the neurological function and reduce the pathological changes after cerebral ischemia in rats. The effect of urokinase on neurological function and pathological changes is not significant.