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目的研究夏星汤药液对人胃癌MGC-803细胞株的增殖、凋亡及p53、bcl-2基因表达的影响。方法采用MTT比色法检测夏星汤药液作用48 h后对MGC-803细胞生长增殖的影响;流式细胞术检测细胞凋亡率;免疫细胞化学法检测p53、bcl-2基因表达。结果随着夏星汤药液浓度逐渐增加,细胞增殖率逐渐降低,细胞抑制率、凋亡率逐渐升高,p53(突变型)和bcl-2蛋白表达得分逐渐下降。结论夏星汤能够抑制人胃癌MGC-803细胞增殖,对细胞有诱导凋亡作用,在凋亡过程中涉及p53(突变型)和bcl-2基因表达下降。
Objective To study the effects of Xiaxing Decoction on proliferation, apoptosis and p53, bcl-2 gene expression in human gastric cancer cell line MGC-803. Methods MTT assay was used to detect the effect of Xia Xing Tang on the proliferation and proliferation of MGC-803 cells 48 h after treatment. The apoptosis rate of MGC-803 cells was detected by flow cytometry. The expressions of p53 and bcl-2 gene were detected by immunocytochemistry. Results As the concentration of Xiaxing decoction increased gradually, the cell proliferation rate decreased gradually, and the cell inhibitory rate and apoptotic rate gradually increased. The expression of p53 (mutant type) and bcl-2 protein decreased gradually. Conclusion Xiaxing decoction can inhibit the proliferation of human gastric cancer cell line MGC-803 and induce cell apoptosis. The expression of p53 (mutant) and bcl-2 gene is decreased during apoptosis.