最近,zhang等从小鼠体内克隆出一种仅在脂肪组织中表达的肥胖控制(obese)基因,OB/?鼠为正常表型,ob/ob鼠为肥胖表型,肥胖的原因是因与肥胖控制基因突异有关,导致其仅表达一截短的无活性蛋白。现在该基因的同源序列也在人类和大鼠中被发现。 本实验用生物合成的方法,在E.coli中分别合成了人和鼠的肥胖控制基因产物,hOB和mOB,经 Recently, zhang et al cloned a obese gene expressed only in adipose tissue from mice. The OB /? Mouse is a normal phenotype and the ob / ob mouse is a fat phenotype. The reason for obesity is due to obesity The control of gene mutations has led to its expression of only a short, inactive protein. The homologous sequences of this gene are now also found in humans and rats. In this experiment, biosynthetic methods were used to synthesize obesity control gene products of human and mouse in E. coli, hOB and mOB respectively