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目的探讨滋阴益气活血解毒方对高脂饮食2型糖尿病转基因MKR小鼠脂肪肝发生中固醇调节元件结合蛋白-1c(SREBP-1c)表达的影响。方法 30只MKR小鼠随机分为对照组、高脂模型组和滋阴益气活血解毒方(中药方)组;高脂模型组和中药方组均以高脂饲料喂养8周,对照组以基础饲料喂养;中药方组高脂饲料喂养8周后,以中药含生药量29.64 g/kg灌胃干预治疗4周,其他2组灌胃等量的蒸馏水;光镜下观察小鼠肝组织脂肪变性,RT-PCR检测肝组织SREBP-1c mRNA表达,免疫组化检测肝组织SREBP-1c蛋白表达。结果高脂模型组小鼠较对照组小鼠肝脏SREBP-1c mRNA和蛋白表达增加,差异具有统计学意义(P<0.01);中药方组降低高脂饮食MKR小鼠肝脏SREBP-1c的mRNA和蛋白表达水平,与高脂模型组比较差异具有统计学意义(P<0.01)。结论滋阴益气活血解毒方通过调节肝脏SREBP-1c表达,缓解糖尿病合并脂肪肝小鼠肝脏脂肪性变。
Objective To investigate the effect of Ziyin Yiqi Huoxue Jiedu Decoction on the expression of steroid regulatory element-binding protein-1c (SREBP-1c) in fatty liver induced by high fat diet in type 2 diabetic MKT mice. Methods Thirty MKR mice were randomly divided into control group, high fat model group and Ziyin Yiqi Huoxue Jiedu Decoction (TCM prescription) group. The high fat model group and the traditional Chinese medicine group were fed with high fat diet for 8 weeks. The control group The rats in the traditional Chinese medicine group were fed with high-fat diet for 8 weeks. The rats in the traditional Chinese medicine group were administrated with 29.64 g / kg gavage for 4 weeks. The other two groups were orally administered with the same amount of distilled water. The expression of SREBP-1c mRNA in liver tissue was detected by RT-PCR and the expression of SREBP-1c in liver tissue was detected by immunohistochemistry. Results Compared with the control group, the expression of SREBP-1c mRNA and protein in the liver increased in the model group and the difference was statistically significant (P <0.01). The Chinese herb group decreased the mRNA and protein expression of SREBP-1c in the liver of the high-fat diet group Compared with high fat model group, the difference of protein expression level was statistically significant (P <0.01). Conclusion Nourishing Yin and Invigorating Qi, Promoting Blood Circulation and Detoxicating Recipe can relieve hepatic steatosis in diabetic mice with fatty liver by regulating the expression of SREBP-1c in liver.