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[目的]研究阿仑瞵酸钠对骨质疏松性骨折愈合过程的影响。[方法]本研究通过切除大鼠双侧卵巢建立骨质疏松模型,并人为造成后肢骨折后,予以阿仑瞵酸钠灌胃,观察骨折愈合过程中骨痂的组织学、骨密度变化,并用免疫组化方法观察TGF-β1、碱性成纤维细胞生长因子、骨形态发生蛋白-2、胰岛素样生长因子的变化。[结果]X线、组织学显示两组骨折同时愈合。两组TGF-β1、碱性成纤维细胞生长因子、骨形态发生蛋白-2、胰岛素样生长因子含量在14-42 d均出现高峰,经过统计学分析,两组差异无统计学意义。骨密度显示自42 d开始实验组与对照组骨折部骨密度有较明显差别。[结论]阿仑瞵酸钠可使钙离子沉积于软骨痂,提高骨密度,对于预防骨质疏松骨折患者发生再骨折是一种良好的方法。但阿仑瞵酸钠对于骨质疏松骨折愈合的过程,既无延缓作用也无促进作用。
[Objective] To study the effect of alendronate on osteoporotic fracture healing. [Method] In this study, osteoporosis models were established by excision of both ovaries in rats and induced by intramuscular injection of alendronate after artificially induced hindlimb fractures. Changes of callus histology and bone mineral density during fracture healing were observed and used Immunohistochemistry was used to observe the changes of TGF-β1, basic fibroblast growth factor, bone morphogenetic protein-2 and insulin-like growth factor. [Results] X-ray and histology showed that both fractures healed simultaneously. The content of TGF-β1, basic fibroblast growth factor, bone morphogenetic protein-2 and insulin-like growth factor in both groups peaked at 14-42 days. After statistical analysis, there was no significant difference between the two groups. Bone mineral density showed a significant difference in bone mineral density between the experimental group and the control group at 42 days. [Conclusion] Sodium alendronate can deposit calcium ion in the cartilage callus to increase the bone density, which is a good method to prevent the recurrence of osteoporosis fracture. However, sodium alendronate for osteoporosis fracture healing process, neither delaying nor promoting role.