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目的:观察大鼠烫伤及补锌对血清、组织中锌(Zn~(2+))、钙(Ca~(2+))离子的影响。方法:背部15%深Ⅱ°烫伤大鼠随机分为N组(正常进食组,饲料含Zn~(2+) 40 μg/g)、W组(创面补锌组,饲料含Zn~(2+)40 μg/g,同时创面涂银锌霜,含Zn~(2+)761.1 μg/g)、H组(口饲补锌组,饲料含Zn~(2+)80 μg/g);分别于伤后1、3、7天活杀大鼠,留取标本检测。结果:烫伤后第1天N组、W组血清Zn~(2+)明显降低,而后回升,伤后7天接近伤前水平;而H组伤后明显升高。N组、W组血清Ca~(2+)伤后降低,H组上升。伤后第1、3天各组肝脏Zn~(2+)均呈上升趋势,以H组最明显(P<0.01)。N、W组肝脏Ca~(2+)均升高,H组低于伤前和N、W组(P<0.01)。烫伤后各组骨Zn~(2+)均呈递减趋势,以N组降低幅度最大,明显低于H组(P<0.01)。伤后N、W组骨Ca~(2+)逐渐下降,N组第7天明显低于H组(P<0.05)。伤后第1天,N、W组烫伤皮肤Zn~(2+)下降,H组明显升高,第3、7天,各组皮肤Zn~(2+)均逐渐上升,以W组最明显。各组烫伤皮肤中Ca~(2+)均呈大幅度递增(P<0.01),其中以N组最明显;W组明显低于N组(P<0.01)。结论:烫伤后血清、骨骼Ca~(2+)含量降低;肝脏、烫伤皮肤Ca~(2+)含量增加,可能与Ca~(2+)体内异常分布、细胞内钙超载有关;口饲补Zn~(2+)主要提高血清、肝脏Zn~(2+),降低肝组织Ca~(2+)含量,创面补Zn~(2+)主要增加烫伤皮肤Zn~(2+),降低烫伤皮肤Ca~(2+)含量,Zn~(2+)可能具有减轻细胞内钙超载的作用。
Objective: To observe the effects of scald and zinc supplementation on the levels of Zn (2+) and Ca (2+) in serum and tissues. METHODS: Fifteen percent deep Ⅱ degree scalded rats on the back were randomly divided into N groups (normal diet group, diet containing Zn 2+ (40 μg / g), W group ) (40 μg / g, meanwhile, the wound was coated with silver-zinc cream containing 761.1 μg / g Zn 2+) and H group Rats were sacrificed on days 1, 3 and 7 after injury, and specimens were taken for detection. Results: On the first day after scalding, the levels of Zn 2+ in group N and group W were significantly lower than those in group W, and then rose back to the level of pre-injury 7 days after injury. N group, W group serum Ca2 + injury decreased, H group increased. On the 1st and 3rd day after injury, the Zn 2+ in the liver of each group showed an upward trend, most notably in the H group (P <0.01). The levels of Ca 2+ in N and W groups were significantly increased, while those in H group were lower than those in N and W groups (P <0.01). After scald, the Zn ~ (2+) in all the groups showed a decreasing trend, the largest decrease in group N was significantly lower than that in group H (P <0.01). In the N and W groups, the Ca2 + level in the W group decreased gradually, and was significantly lower in the N group than in the H group on the 7th day (P <0.05). On the first day after injury, Zn 2+ in scalded skin of N and W groups decreased significantly, while H group increased significantly. On the 3rd and 7th day, Zn 2+ in the skin of all groups gradually increased, which was the most obvious in W group . The contents of Ca ~ (2+) in scalded skin of all the groups increased significantly (P <0.01), of which the most obvious was in group N; the level of Ca in group W was significantly lower than that in group N (P <0.01). Conclusion: The content of Ca2 + in serum and bone decreased after scald, the content of Ca2 + in liver and scalded skin increased, which may be related to the abnormal distribution of Ca2 + and the overload of intracellular calcium. Zn 2+ mainly increased Zn 2+ in serum and liver, reduced the content of Ca 2+ in liver tissue, increased Zn 2+ in wound surface and decreased the scald Skin Ca 2+ content, Zn 2+ may have the effect of reducing intracellular calcium overload.