Double strand breaks (DSBs) are the most detrimental type of DNA damage that must be repaired to ensure genome integrity and cell survival.Unrepaired or improperly repaired DSBs can potentially cause tumorigenesis or cell death.DSBs are primarily repaired by non-homologous end joining or homologous recombination (HR).The HR pathway is initiated by processing of the 5’-end of DSBs to generate 3’-end single-strand DNA (ssDNA).Furthermore,the intermediate is channeled to one of the HR sub-pathways,including:(i) double Holliday junction (dHJ) pathway,(ii) synthesis-dependent strand annealing (SDSA),(iii) break-induced replication (BIR),and (iv) singlestrand annealing (SSA).In the dHJ sub-pathway,the 3’-ssDNA coated with Rad51 recombinase performs homology search and strand invasion,forming a displacement loop (D-loop).Capture of the second end by the D-loop generates a dHJ intermediate that is subsequently dissolved by DNA helicase or resolved by nucleases,producing non-crossover or crossover products.In SDSA,the newly synthesized strand is displaced from the D-loop and anneals to the end on the other side of the DSBs,producing non-crossovers.In contrast,BIR repairs one-end DSBs by copying the sequence up to the end of the template chromosome,resulting in translocation or loss of heterozygosity.SSA takes place when resection reveals flanking homologous repeats that can anneal,leading to deletion of the intervening sequences.A variety of reporter assays have been developed to monitor distinct HR sub-pathways in both Saccharomyces cerevisiae and mammals.Here,we summarize the principles and representative assays for different HR sub-pathways with an emphasis on the studies in the budding yeast.