论文部分内容阅读
作者用单克隆抗体VIMD5(CD15单抗,识别出现在早幼粒到粒细胞的分化抗原)及MY10(CD34单抗,与前体细胞反应,仅极少(<5%)正常骨髓细胞有反应).在16例未经化疗的初发AML的白血病细胞进行表面标志,并用流式细胞术同时检测DNA含量,以Baisch氏法(I式)从图象中算出各细胞周期细胞的百分率.结果:全部病例均表达CD34(11-80%白血病细胞染色),CD15表达者14例(16-50%细胞).细胞周期分析:CD34~+细胞百分率与S+G2+M细胞的百分率有关联(P<0.05),而CD15表达与S+G2+M之间无关联.CD34~+的S+G2+M期细胞占的
The authors used the monoclonal antibody VIMD5 (CD15 monoclonal antibody that recognizes differentiation antigens that appear in promyelocytic and granulocyte) and MY10 (CD34 monoclonal antibody, which reacts with precursor cells and responds only rarely (<5%) to normal bone marrow cells. ). Sixteen untreated leukemia cells that had not been treated with chemotherapy were subjected to surface labeling, and DNA content was measured simultaneously by flow cytometry. The percentage of cells in each cell cycle was calculated from the images using the Bais’s method (I). : All cases expressed CD34 (11-80% leukemia cell stained), CD15 expressed 14 cases (16-50% cells). Cell cycle analysis: The percentage of CD34~+ cells is related to the percentage of S+G2+M cells ( P<0.05), but there was no correlation between CD15 expression and S+G2+M. The proportion of CD34+ S+G2+M cells accounted for