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目的:探讨人参皂苷Rg1对体外胚鼠皮层神经干细胞的增殖和神经胶质定向分化的影响。方法:本研究采用MTT法测定人参皂苷Rg1对胚鼠皮层神经干细胞的最佳剂量和最佳作用时间点,氧糖剥夺(OGD)后的细胞损伤模型,免疫荧光染色鉴定神经干细胞增殖和神经胶质样定向分化。结果:分离后体外培养的胚鼠皮层细胞表达Nestin并自我复制,为神经干细胞,人参皂苷Rg1对神经干细胞增殖的最佳剂量是0.016μg/m L,0.016μg/m L人参皂苷Rg1对神经干细胞的增殖最佳作用时间点是6h。在人参皂苷Rg1的作用下,神经干细胞及OGD后的神经干细胞中Nestin、Brd U、光密度、阳性细胞Vimentin的面密度数目及Nestin/Brd U、Nestin/Vimentin双标染色细胞数目有显著提高(P<0.05,P<0.01)。结论:人参皂苷Rg1具有促进皮层神经干细胞增殖和神经胶质样定向分化的作用,这一机制可能是人参皂苷Rg1中具有类似于生长因子或刺激某些细胞分泌生长因子,促进神经干细胞的胶质样分化和增殖。
Objective: To investigate the effects of ginsenoside Rg1 on the proliferation and glial differentiation of neural stem cells in vitro. Methods: MTT assay was used to determine the best dose and time point of ginsenoside Rg1 on embryonic rat cortical neural stem cells, cell injury model after OGD, immunofluorescence staining to identify neural stem cell proliferation and neural glue Quality-oriented differentiation. Results: Nestin was isolated and cultured in vitro. The optimal dosage of ginsenoside Rg1 for proliferation of neural stem cells was 0.016 μg / mL. Nestin, 0.016 μg / mL, did not affect the proliferation of neural stem cells The best time to proliferate is 6h. Under the action of ginsenoside Rg1, the numbers of Nestin, BrdU, optical density, the number of Vimentin positive cells and the numbers of Nestin / BrdU and Nestin / Vimentin double-labeled cells in neural stem cells and OGD-derived neural stem cells were significantly increased P <0.05, P <0.01). CONCLUSION: Ginsenoside Rg1 can promote the proliferation and glial-like differentiation of neural stem cells in vitro. The mechanism may be that ginsenoside Rg1 has similar growth factors or stimulates the secretion of growth factors in certain cells and promotes glial cells of neural stem cells Like differentiation and proliferation.