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亲代小鼠分别喂食转基因和非转基因大豆饲料,120 d后组内交配获得子一代小鼠。以转基因饲料喂食的亲代所繁殖的子代小鼠作为实验组,断乳后子鼠继续喂食转基因大豆饲料;对照组子鼠为亲代非转基因喂食组所繁殖,断乳后继续喂食非转基因大豆饲料。分别于喂食30、60、90、120 d取子代小鼠的睾丸称重并计算脏器系数,伊红法染色检测精子存活率与畸形率;制作石蜡切片HE染色后观察睾丸病理学变化。结果表明:与对照组相比,实验组小鼠睾丸系数、精子存活率与畸形率均无显著性差异;组织切片镜检未发现两组小鼠的睾丸出现明显病理损伤。实验结果说明转基因大豆饲料喂食120 d未对子代雄鼠精子质量和睾丸组织病理造成影响,表明亲代长期喂食转基因大豆饲料后无潜在遗传毒性及积累效应。
The parental mice were fed genetically modified and non-GMO soybean feed, respectively, and the first generation mice were mated after 120 days. The transgenic offspring of the offspring of the offspring of the offspring mice were used as the experimental group, the weanling offspring continued to feed the transgenic soybean feed; control group of offspring of the parental non-transgenic feeding group breeding, weaning continue to feed non-genetically modified soybean feed . The testes of the offspring mice were weighed at 30, 60, 90 and 120 days after feeding respectively, and the organ coefficient was calculated. The survival rate and deformity rate of sperm were detected by eosin staining. The pathological changes of testis were observed after HE staining. The results showed that compared with the control group, there was no significant difference in testis coefficient, sperm survival rate and deformity rate in the experimental group; no significant pathological damage was observed in the testes of the two groups. The results showed that transgenic soybean feed did not affect the sperm quality and testicular histopathology of the male offspring for 120 days, indicating that there is no potential genotoxicity and accumulation effect after long-term feeding of transgenic soybean feed.