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目的分析reelin基因突变小鼠,即reeler小鼠海马锥体细胞和颗粒细胞的组织化学特征,了解Reelin缺乏对海马皮质发育的影响,为认识Reelin功能提供形态学证据。方法用免疫荧光双重标记法,分别标记野生型和reeler小鼠海马锥体细胞、颗粒细胞和苔藓细胞;结果Reeler小鼠海马皮质板发育明显障碍,表现为锥体层和颗粒层细胞扩散,海马片层状细胞构筑紊乱。颗粒层细胞明显增殖并向门区迁移,以致颗粒层与门区的界限消失,呈鼓槌状结构。结论Reelin作为神经细胞迁移的终止信号和细胞增殖的调控信号对神经细胞迁移、发育过程中皮质板的片层化,特别是对调节颗粒细胞的增殖有明显影响。
Objective To analyze the histochemical characteristics of hippocampal pyramidal cells and granulosa cells of reelin mutant mice, and to understand the influence of reelin deficiency on hippocampal cortical development and to provide morphological evidence for understanding Reelin function. Methods The hippocampal pyramidal cells, granulosa cells and moss cells of wild-type and reeler mice were labeled by immunofluorescence double labeling method. Results The hippocampal cortical plate of Reeler mice developed obvious obstacles, which manifested as the proliferation of pyramidal layer and granulosa layer, hippocampus Layered cells build disorder. Granulosa cells significantly proliferate and migrate to the gate, resulting in the boundaries of the granular layer and the door disappeared, was drumstick-like structure. Conclusion Reelin, as the signal of the termination of neural cell migration and the regulator of cell proliferation, has a significant effect on the migration of neural cells and the lamellarization of cortical plate during development, especially on the regulation of the proliferation of granulosa cells.