论文部分内容阅读
目的:基于代谢物组学理论,探讨黄连解毒汤的拆方黄连-黄芩及黄连-黄芩-黄柏干预2,4-二硝基苯酚诱导热病证候模型在代谢物组学层面上的关联性。方法:运用UPLC/MS技术手段,采用PCA和PLS-DA数据解析方法,研究黄连-黄芩及黄连-黄芩-黄柏对于热病证候模型大鼠尿液中生物标记物的影响。结果:初步确定2,4-二硝基苯酚诱导热病证候模型51个生物标志物,黄连-黄芩对于其中8个生物标志物具有明显干预作用,黄连-黄芩-黄柏对于其中15个生物标志物有明显干预作用。结论:黄连-黄芩-黄柏对于热病证候模型生物标志物的干预数量和程度均好于黄连-黄芩,佐药黄柏的加入有效加强了对热病的治疗作用,从代谢物组学的角度阐明了传统复方理论中“君臣佐使”组方原则的科学性。
OBJECTIVE: To investigate the association of Coptis chinensis-Scutellaria baicalensis Georgi-Radix Scutellariae-Scutellaria baicalensis Georgi . Methods: The effects of berberine - Scutellaria baicalensis Georgi - Radix Scutellariae - Cortex Phellodendri on the biomarkers in urine of rats with fever syndrome were studied by UPLC / MS and PCA and PLS-DA data analysis methods. Results: Totally 51 biomarkers of heat syndrome induced by 2,4-dinitrophenol were identified. Coptis-Scutellariae had obvious intervention effects on 8 biomarkers. Coptis-Scutellariae-Cortex Phellodendri for 15 biomarkers There are obvious interventions. CONCLUSION: The quantity and degree of intervention of berberine - Scutellaria baicalensis - Phellodendri for biomarkers of fever syndrome model are better than those of Coptis - Scutellariae, and the addition of adjuvant Phellodendron cortex effectively strengthens the therapeutic effect on fever, and from the perspective of metabolomics Clarified the scientific nature of the principle of the “royal monarchy” in the traditional compound theory.