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经口一次投与不同浓度氟化钠(0、15、50mg/kg)后大鼠血清F浓度、肾组织学及酶组织化学动态变化结果表明,肾脏病变与投氟量或血氟浓度呈明显的剂量依赖关系,氟主要损害肾皮质外带和皮一髓质交界处的近曲小管,表现为上皮细胞肿胀、空泡变性及坏死脱落。近曲小管上皮细胞膜性Na~+/K~+—ATPase活性减弱,细胞出现水样变性,同时其他膜结合酶(Ca~(+2)—ATPase与ALP)、内质网酶(ANAE)及溶酶体酶(ACP)活性也显著降低,表明氟对膜Na~+/K~+—ATPase等的抑制及与此有关的细胞损伤是氟中毒时肾功能改变的生化病理基础.
The changes of serum F concentration, renal histology and enzyme histochemistry in rats after oral administration of different concentrations of sodium fluoride (0, 15 and 50 mg / kg) once a day showed that the changes of renal lesion, In a dose-dependent manner, fluorine mainly damaged proximal convoluted tubules in the junction of renal cortex and dermal-medulla, showing epithelial cell swelling, vacuolar degeneration and necrosis. The membrane Na + / K + -ATPase activity of proximal tubule epithelial cells was weakened, and the cells showed watery degeneration. At the same time, other membrane-associated enzymes (Ca ~ (+2) -ATPase and ALP), ANAE Lysosomal enzyme (ACP) activity was also significantly reduced, indicating that fluoride on the membrane Na ~ + / K ~ + -ATPase inhibition and cell damage associated with fluorosis is the change of renal function biochemical pathology.