论文部分内容阅读
为了在较低剂量下研究胰岛素栓的降血糖作用,本文应用各种表面活性剂制备栓剂,研究了这些表面活性剂对胰岛素直肠吸收的影响。所用表面活性剂有阴离子型、阳离子型、非离子型与两性离子型,还有胆酸与卵磷脂。将胰岛素溶解或混悬于0.1M枸橼酸盐缓冲液或0.1M磷酸盐缓冲液中制成pH_3～8的各种溶液;用日本玉米油作基质,混入备种浓度的各种表面活性剂制备胰岛素检;将200U胰岛素锌制成100ml注射液,溶媒为1.4%W/V甘油、苯酚0.1%W/V与0.01M盐酸。用雄兔(2.0～2.4kg)作试验动物,实验前24小时禁食使直肠内的粪便完全排空。用玻璃注射管(长7.5cm、
In order to study the hypoglycemic effect of insulin suppositories at lower doses, various surfactants were used to prepare suppositories, and the effect of these surfactants on the rectal absorption of insulin was investigated. Surfactants used are anionic, cationic, nonionic and zwitterionic, as well as bile acids and lecithin. Insulin was dissolved or suspended in 0.1 M citrate buffer or 0.1 M phosphate buffer to prepare various solutions of pH_3 to 8; with Japanese corn oil as a matrix, various concentrations of prepared surfactants were mixed Insulin preparation was performed; 200 U insulin zinc was made into a 100 mL injection at 1.4% W / V glycerol, 0.1% W / V phenol and 0.01 M hydrochloric acid. Male rabbits (2.0-2.4 kg) were used as experimental animals, and fasted for 24 hours before the experiment to completely empty the rectum. Glass injection tube (length 7.5cm,