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目的 :探讨实验性变态反应性脑脊髓炎 (EAE)时 ,中枢和外周儿茶酚胺水平的变化规律 ,为神经系统自身免疫性疾病的发病机制和治疗的进一步研究提供实验依据。方法 :用豚鼠脊髓加完全弗氏佐剂制成抗原佐剂乳化物诱导Wistar大鼠发生EAE。在EAE 2级和 3级临床症状时 ,用高效液相色谱 电化学检测法 (HPLC ECD)测定下丘脑、海马、肠系膜淋巴结和血清中去甲肾上腺素 (NA)、多巴胺 (DA)和肾上腺素 (A)的含量。结果 :①诱导大鼠EAE ,一般在注射抗原佐剂乳化物两周后开始出现症状 ,2级症状为后肢无力 ,3级症状为后肢瘫痪 ;②EAE 2级和3级症状时 ,下丘脑和海马中NA含量都明显降低 ,而肠系膜淋巴结中NA含量显著升高 ,且症状越重 ,NA含量变化越大 ;③ 3级症状的EAE大鼠 ,下丘脑、海马和淋巴结中DA含量都显著增加 ,而在 2级症状时 ,只有淋巴结中DA含量明显增加 ;④ 2级症状的EAE大鼠 ,血清中A含量高于正常大鼠近 2 .5倍 ,3级症状的EAE大鼠 ,血清中A含量高于正常近 3倍。EAE大鼠淋巴结中A含量也升高 ,但升高幅度没有血清中的大。结论 :大鼠EAE疾病时 ,主要伴有中枢NA降低 ,外周儿茶酚胺升高 ,且EAE症状越重 ,儿茶酚胺含量的变化越大 ,这可能有利于EAE疾病的恢复。
OBJECTIVE: To investigate the changes of catecholamine levels in central and peripheral areas of experimental allergic encephalomyelitis (EAE) and provide experimental evidence for the further study on the pathogenesis and treatment of nervous system autoimmune diseases. Methods: EAE was induced in Wistar rats by using guinea pig spinal cord plus complete Freund ’s adjuvant to make antigen adjuvant emulsion. At the EAE level 2 and 3 clinical symptoms, norepinephrine (NA), dopamine (DA) and epinephrine in the hypothalamus, hippocampus, mesenteric lymph nodes and serum were determined by high performance liquid chromatography electrochemical detection (HPLC ECD) (A) content. Results: ① EAE was induced in rats two weeks after the injection of antigen adjuvant emulsion. The symptom of grade 2 was hindlimb weakness and the grade 3 symptom was paraplegia of hind limb. ② In the case of grade 2 and 3 of EAE, hypothalamus and hippocampus The content of NA was significantly decreased, while the content of NA in mesenteric lymph nodes was significantly increased, and the more the symptoms were, the greater the change of NA content was. ③The content of DA in grade 3 EAE rats, hypothalamus, hippocampus and lymph node were significantly increased, While in grade 2 symptoms, only the content of DA in the lymph nodes increased significantly. (4) The level of A in grade 2 EAE rats was higher than that in normal rats The content is nearly 3 times higher than normal. The content of A in lymph nodes of EAE rats also increased, but the increase was not as large as in serum. CONCLUSIONS: In rats with EAE disease, there is a decrease in central NA and peripheral catecholamines, and the more severe the EAE, the greater the change in catecholamine levels, which may be beneficial to the recovery of EAE disease.