论文部分内容阅读
目的：研究胸腺五肽及其衍生肽在体外对不同细菌的杀菌活性。方法：根据胸腺五肽结构分别设计合成4种衍生肽如下：①胸腺五肽P5；②直肽链P10；③直肽链P12；④环肽P环。采用琼脂铺板计数法，测定衍生肽的杀菌活性。结果：P5、P10、P12对G+菌和G-菌有一定杀菌活性，其杀菌活性由强到弱依次为P12>P10=P5，P环没有杀菌活性。结论：胸腺五肽及其衍生肽P5、P10、P12对G+菌和G-菌有较弱的杀菌活性，P12环经过修饰成环状后，无杀菌活性，这可能与其疏水性和分子结构有关系。“,”Objective To study the bactericidal activity of Thymopentin peptide and its derived peptides in vitro.Methods According to the amino acid sequence of Thymopentin,four peptides were synthesized respectively as fellow:①Thymopentin P5;②Straight peptide P10;③Straight peptideP12;④Ring peptide Pring,.The bactericidal activity was determined by agar plating-method.Results P5、P10、P12 possessed bactericidal activity against G+and G-bacteria,its bactericidal activity from strong to weak was P12>P10=P5. Pringhave no bactericidal activity.Conclusion Thymopentin peptide and its derived peptides P5、P10、P12 possess weak bactericidal activity against G+and G- bacteria .Pringhave no bactericidal activity.It’s bactericidal activity is related to hydrophobicity in the peptide molecule.