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目的了解甘肃省武威市婴幼儿腹泻患者中病毒性腹泻病原体的分布状况。方法收集2011年1月至2012年12月武威市凉州医院住院确诊腹泻婴幼儿的基本信息及其粪便标本,采用酶联免疫吸附试验和反转录-聚合酶链反应(RT-PCR)法检测轮状病毒、星状病毒、肠道腺病毒、杯状病毒。结果 630份粪便标本中4种腹泻病毒总的检出率为68.4%(431/630)、女性婴幼儿腹泻病毒检出率75.0%(147/196)显著高于男性婴幼儿65.4%(284/434)。2012年腹泻病毒检出率73.1%(226/309)显著高于2011年检出率63.9%(205/321)。腹泻病毒检测阳性率依次为杯状病毒34.6%(218/630),轮状病毒34.0%(214/630)、星状病毒13.0%(82/630)、肠道腺病毒10.6%(67/630)。431份检出腹泻病毒的样本中有124份为混合感染,占28.8%(124/431),2012年腹泻病毒混合感染所占比例35.0%(79/226)显著高于2011年腹泻病毒所占比例22.0%(45/205)。214份轮状病毒阳性的标本中病毒毒株G分型以G3,G1为主,P分型以P8,P4为主。结论武威市婴幼儿病毒性腹泻的主要病原体是杯状病毒和轮状病毒。
Objective To understand the distribution of viral diarrhea pathogens in infant diarrhea in Wuwei City, Gansu Province. Methods The basic information and stool samples of infants and young children diagnosed as diarrhea admitted to Liangzhou Hospital of Wuwei City from January 2011 to December 2012 were collected. Enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) Detection of rotavirus, astrovirus, enteric adenovirus, calicivirus. Results The total detection rate of the four diarrhea virus in 630 stool specimens was 68.4% (431/630) and the prevalence of infant diarrhea virus in women was 75.0% (147/196), significantly higher than that of male infants (65.4%) (284 / 434). The detection rate of diarrhea virus in 2012 was 73.1% (226/309), significantly higher than the detection rate of 63.9% (205/321) in 2011. The positive rates of diarrhea virus were 34.6% (218/630) for calicivirus, 34.0% (214/630) for rotavirus, 13.0% (82/630) for astrovirus and 10.6% (67/630) for enteric adenovirus ). Of the 431 samples with diarrhea virus detected, 124 were mixed, accounting for 28.8% (124/431). In 2012, 35.0% (79/226) of the diarrhea virus mixed infection was significantly higher than that of the 2011 diarrhea virus The proportion of 22.0% (45/205). 214 strains of rotavirus positive strains of virus strains G G3, G1-based, P-type to P8, P4-based. Conclusion The main pathogens of infantile viral diarrhea in Wuwei City are calicivirus and rotavirus.