测定油菜籽硫甙总量的内源酶法．其原理是依据内源硫甙酶水解硫甙，以３，５一二硝基水杨酸为显色剂，测定水解产物葡萄糖量，再换算成硫甙总量。４０％的酸化甲醇可完全抑制硫甙酶活性．方法简单、快速，精密度高，重现性好。葡萄糖回收率为９３．６４％±１．３７％。 Endogenous enzyme assay for the determination of the total amount of rapeseed glucosinolate. The principle is based on endogenous glycosaminoglycan hydrolysis glucosinolate, 3,5-dinitrosalicylic acid as a chromogenic reagent, determination of the amount of glucose hydrolysates, and then converted into total glucosinolate. Forty percent of acidified methanol completely inhibits glucosidase activity. The method is simple, fast, high precision, good reproducibility. The recovery of glucose was 93.64% ± 1.37%.