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目的:探讨三黄泻心汤对重型颅脑损伤大鼠胃组织核因子-κB(NF-κB)、白细胞介素-6(IL-6)表达的影响。方法:Wistar大鼠70只(雄雌各半),随机分为正常对照组10只,模型组30只,中药组30只,后2组再按应激后6,24,72 h 3个时相点平均分为3个亚组,每组各10只,除正常对照组外,其余各组予自由落体撞击法建立大鼠重型颅脑损伤模型,分别在造模后6,24,72 h 3个时相点取胃组织。HE染色行胃组织病理学,并用免疫组化法检测不同时相点胃组织NF-κB及IL-6的表达。结果:正常对照组大鼠胃黏膜表层上皮结构完整,腺体排列整齐;模型各组大鼠胃黏膜出现缺损,附近血管扩张,炎症细胞浸润,形成表浅糜烂;中药组胃黏膜细胞损伤程度和毛细血管及其周围间质的水肿减轻,以72 h组最明显。IL-6,NF-κB着色于胞浆,在重型颅脑损伤大鼠各组胃组织中均有表达。与正常对照组比较,模型24,72 h组大鼠IL-6,NF-κB的表达明显增加(P<0.01～0.05);与模型24 h组比较,中药24 h组大鼠胃组织IL-6的表达含量显著降低(P<0.05);与模型72 h组比较,中药72h组大鼠损伤区皮质IL-6,NF-κB的表达含量显著降低(P<0.01～0.05)。结论:三黄泻心汤抑制重型颅脑损伤大鼠胃组织核因子NF-κB,IL-6的表达可能是其防治重型颅脑损伤急性胃黏膜病变保护作用的机制之一。
Objective: To investigate the effect of San Huang Xie Xin Tang on the expression of nuclear factor-κB (NF-κB) and interleukin-6 (IL-6) in the stomach of rats with severe craniocerebral injury. Methods: Seventy male and female Wistar rats were randomly divided into normal control group (n = 10), model group (n = 30) and traditional Chinese medicine group (n = 30) The average points were divided into 3 subgroups, 10 in each group. Except for the normal control group, the rats in each group were given free-fall impact model to establish the model of severe traumatic brain injury in rats. After 6, 24, 72 h Three time points to take the stomach tissue. The gastric histopathology was performed by HE staining. The expressions of NF-κB and IL-6 in gastric tissues at different time points were detected by immunohistochemistry. Results: The epithelial structure of gastric mucosa of rats in normal control group was intact and the glands were arranged neatly. The gastric mucosa of the rats in each model group showed defects, vasodilation and infiltration of inflammatory cells in the model group, resulting in superficial erosion. The edema of the capillaries and their surrounding stroma was alleviated, most notably in the 72 h group. The expression of IL-6 and NF-|ÊB in the cytoplasm was observed in all groups of rats with severe traumatic brain injury. Compared with the normal control group, the expressions of IL-6 and NF-κB in the 24 and 72 h groups were significantly increased (P <0.01 ~ 0.05). Compared with the 24 h group, the levels of IL- (P <0.05). Compared with the model group at 72 h, the expressions of IL-6 and NF-κB in the cortex of rats in the 72 h group were significantly decreased (P <0.01-0.05). Conclusion: Inhibition of Sanhuang Xiexin Decoction on the expression of NF-κB and IL-6 in gastric tissue of rats with severe craniocerebral injury may be one of its mechanisms in the prevention and treatment of acute gastric mucosal lesion in severe craniocerebral injury.