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目的掌握2009年邯郸市0～15岁儿童EV71IgG和CoxA16IgG携带状况,探讨发病率与抗体的关系。方法采用酶联免疫吸附试验(ELISA)检测0～15岁儿童血清EV71IgG和CoxA16IgG,分析EV71IgG、CoxA16IgG阳性率随年龄的变化趋势及其与手足口病发病率间的关系。结果 2009年邯郸市报告HFMD病例19 457例,567例HFMD病例标本中450份肠道病毒核酸阳性,其中EV71 302份(占67.11%),CoxA16 75份(占16.67%)。采集856名儿童血清,EV71IgG阳性546例,阳性率63.79%;CoxA16IgG阳性579例,阳性率67.64%,差异无统计学意义(χ2=1.309,P>0.05)。儿童血清EV71IgG和CoxA16IgG阳性率呈负相关(r=-0.900),5岁以下儿童血清EV71IgG阳性率与2009年HFMD发病率呈正相关关系(r=0.852),CoxA16IgG阳性率与发病率呈负相关关系(r=-0.816)。结论 2009年邯郸市CoxA16和EV71均有流行,病例以EV71型为主。
Objective To understand the status of EV71IgG and CoxA16IgG in 0-15-year-old children in Handan City in 2009 and explore the relationship between the incidence and antibody. Methods Serum EV71IgG and CoxA16IgG were detected by enzyme-linked immunosorbent assay (ELISA) in children aged 0-15 years. The positive rates of EV71IgG and CoxA16 IgG were analyzed with age and their relationship with the incidence of HFMD. Results In 2009, 19 457 HFMD cases were reported in Handan City. Of the 567 HFMD cases, 450 were positive for enterovirus nucleic acid, of which 302 were EV71 (67.11%) and 75 were CoxA16 (16.67%). A total of 856 children were enrolled in this study. The positive rate of EV71 IgG was 54.7%, and the positive rate was 63.79%. The positive rate of CoxA16 IgG was 579, the positive rate was 67.64%. There was no significant difference (χ2 = 1.309, P> 0.05). The positive rates of EV71 IgG and CoxA16 IgG in children were negatively correlated (r = -0.900). The positive rates of serum EV71 IgG in children under 5 years old were positively correlated with the incidence of HFMD in 2009 (r = 0.852). The positive rate of CoxA16 IgG was negatively correlated with the incidence (r = -0.816). Conclusion In 2009, CoxA16 and EV71 in Handan City were both endemic. The patients were mainly EV71.