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利用中抗 (15 0 6倍 )品系 ,室内用氰戊菊酯对棉铃虫Helicoverpaarmigera初孵至 4龄幼虫分别进行连续汰选 ,选育 9代后 ,以 3龄幼虫汰选的抗性发展最快 (31 5倍 ) ,其次是 4龄、 2龄幼虫汰选的品系 (分别增加 2 5 2倍和 14 5倍 ) ,用初孵幼虫汰选的抗性发展最慢 (10 2倍 )。抗性现实遗传力的测定表明 ,3龄幼虫汰选的抗性现实遗传力 (0 4 4 19)显著大于初孵幼虫的(0 2 346 )。代谢酶抑制剂的增效实验发现 ,磷酸三苯酯 (TPP)对各品系棉铃虫均无明显增效作用。而增效醚 (PBO)对高龄幼虫汰选的品系的增效作用比低龄幼虫汰选的品系增效作用强。测定初孵和 3龄幼虫汰选品系试虫的击倒抗性发现 ,初孵幼虫汰选品系的抗性增加倍数 (10 2 )与击倒抗性增加的倍数 (10 5)相似 ,而 3龄幼虫汰选的抗性增加倍数 (31 5)显著高于击倒抗性增加的倍数 (19 9)。认为初孵幼虫期多功能氧化酶 (MFO)表达不完全 ,用药主要是筛选击倒抗性 ,而高龄幼虫期用药则会同时筛选击倒抗性和MFO参与的代谢抗性。因而初孵幼虫期用药抗性发展缓慢。生产上不仅可以提高药剂的防效 ,同时可以延缓抗性的发展。
With mid-resistance (1506-fold) lines, fenvalerate was used to eliminate Helicoverpa armigera newly-hatched to 4th-instar larvae respectively. After 9 generations of selection, the resistance to 3rd-instar larvae (31 5-fold), followed by the 2nd and 4th instar larvae (25.5 and 14.5 fold, respectively). The resistance to the newly hatched larvae was the slowest (102%). The resistance heritability test showed that the resistance heritability of the 3rd instar larvae (0 4 4 19) was significantly greater than that of the newly hatched larvae (0 2 346). The synergistic experiments of metabolic enzyme inhibitors found that triphenyl phosphate (TPP) had no synergistic effect on each strain of cotton bollworm. The synergistic effects of PBO on the selected strains of older larvae were stronger than that of the strains selected on the younger larvae. The resistance to knock-out of the newly hatched and 3rd instar larvae selection strains was found to be similar (10 2) to the fold increase (10 5) in the newly hatched larvae, The fold increase (31 5) of age-selected larvae was significantly higher than that of knock-down resistance (19 9). It is considered that the expression of multifunctional oxidase (MFO) in newly hatched larvae is not fully expressed, and the drug is mainly used to screen the knockdown resistance, while the aged larvae use both the knockdown resistance and the metabolic resistance involved in the MFO. Therefore, the newly hatched larvae of drug resistance slow development. Production can not only improve the anti-drug efficacy, but also can delay the development of resistance.