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目的:探讨运动对高蛋氨酸饮食大鼠血浆6-酮-前列腺素F1α(6-Keto-PGF1α)、血栓素B2(TXB2)含量和PGI2/TXA2系统的影响。方法:雄性Wistar大鼠随机分为正常饮食对照组(C组)、高蛋氨酸饮食组(M组)和有氧运动+高蛋氨酸饮食组(M+T组)。M组和M+T组大鼠喂饲含3%高蛋氨酸的高蛋氨酸饲料,M+T组大鼠每日同时进行90min无负重游泳运动,每周6天。C组喂饲普通饲料,实验共8周。采用高效液相色谱法测定实验后各组大鼠血浆同型半胱氨酸(Hcy)含量、放射免疫非平衡法测定血栓素B2(TXB2)和6-酮-前列腺素F1α(6-Keto-PGF1α)含量。结果:M组血浆Hcy含量显著高于C组(P<0.01),是C组的2倍以上,6-Keto-PGF1α/TXB2比值显著下降(P<0.01)。与M组相比,M+T组血浆Hcy显著下降(P<0.05),血浆6-Keto-PGF1α含量显著提高,TXB2含量显著下降(P<0.01),6-Keto-PGF1α/TXB2比值显著提高(P<0.05),且与C组相比上述各项指标无显著差异。结论:高蛋氨酸饮食可诱发大鼠高半胱氨酸血症,血浆PGI2/TXA2失衡;适宜的运动可以降低高蛋氨酸饮食大鼠血浆Hcy水平,改善PGI2/TXA2失衡,预防高半胱氨酸血症形成。
Objective: To investigate the effects of exercise on 6-Keto-PGF1α, TXB2 and PGI2 / TXA2 in rats with high methionine diet. Methods: Male Wistar rats were randomly divided into normal diet control group (C group), high methionine diet group (M group) and aerobic exercise + high methionine diet group (M + T group). Rats in M group and M + T group were fed with high methionine diet containing 3% of high methionine. Rats in M + T group were subjected to non-weight-bearing swimming for 90 minutes daily for 6 days. C group fed normal feed, the experiment a total of 8 weeks. The content of plasma homocysteine (Hcy) in each group was measured by high performance liquid chromatography (HPLC), and the levels of TXB2 and 6-Keto-PGF1α )content. Results: The plasma Hcy level in group M was significantly higher than that in group C (P <0.01), which was more than twice as high as that in group C, and the ratio of 6-Keto-PGF1α / TXB2 was significantly decreased (P <0.01). Compared with M group, Hcy in M + T group was significantly decreased (P <0.05), plasma 6-Keto-PGF1α content was significantly increased, TXB2 content was significantly decreased (P <0.01), 6-Keto-PGF1α / TXB2 ratio was significantly increased (P <0.05), and there was no significant difference between the above indexes in C group. CONCLUSION: High methionine diet can induce homocysteinemia and PGI2 / TXA2 imbalance in plasma. Appropriate exercise can reduce plasma homocysteine level, improve PGI2 / TXA2 imbalance and prevent homocysteine in rats Symptoms.